Phenotype Image Detail

Image

Caption

Brca2^tm1Brn/Brca2^tm1Brn Tg(Pbsn-cre)4Prb/0 (Brca2^F/F;PBCre4), Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53⁺ Tg(Pbsn-cre)4Prb/0 (Brca2^F/F;Trp53^F/+;PBCre4), and Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0 (Brca2^F/F;Trp53^F/F;PBCre4) mutants have increased DNA damage and apoptosis. A) Immunohistochemistry for gammaH2AX shows several cells have DNA damage in low-grade prostate intraepithelial neoplasia (LG PIN) areas in Brca2^tm1Brn/Brca2^tm1Brn Tg(Pbsn-cre)4Prb/0 and Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53⁺ Tg(Pbsn-cre)4Prb/0 prostates, whereas control (Brca2^F/F) prostates have undetectable levels of DNA damage. Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0 mice have multi-focal areas with a large number of cells with DNA damage in regions of HG PIN. (B) TUNEL assay on sections of prostates demonstrates Brca2^tm1Brn/Brca2^tm1Brn Tg(Pbsn-cre)4Prb/0 and Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53⁺ Tg(Pbsn-cre)4Prb/0 mutant prostates have an increased level of apoptosis, compared to control prostates. The level of apoptotic cells increases further in HG PIN lesions of Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0 mutants. Arrows indicate apoptotic cells. The anterior prostates of 16-month-old animals are shown.

Copyright

This image is from Francis JC, PLoS Genet 2010;6(6):e1000995, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:161511

Associated Alleles

Symbol	Name
Brca2^tm1Brn	breast cancer 2, early onset; targeted mutation 1, Anton Berns
Tg(Pbsn-cre)4Prb	transgene insertion 4, Pradip Roy-Burman
Trp53^tm1Brn	transformation related protein 53; targeted mutation 1, Anton Berns

Associated Genotypes

Allelic Composition	Genetic Background
Brca2^tm1Brn/Brca2^tm1Brn Tg(Pbsn-cre)4Prb/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2
Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^tm1Brn Tg(Pbsn-cre)4Prb/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2
Brca2^tm1Brn/Brca2^tm1Brn Trp53^tm1Brn/Trp53^+ Tg(Pbsn-cre)4Prb/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2