Phenotype Image Detail

Image

Caption

Conditional inactivation of Neurog3 in only the intestine results in a complete loss of all enteroendocrine cells all along the proximal-distal axis of the intestine. Sections of adult duodenum, jejunum, ileum, and colon were examined for the presence of endocrine cells in control and Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 or Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 (mutant) animals by immunofluorescence. Images presented are from the jejunum of control (A, C, E, G, and I) and mutant (B, D, F, H, and J) animals and are representative for the general loss of all enteroendocrine cells in mutant animals. Villin-Cre-mediated inactivation of Neurog3 results in a complete loss of all Neurog3+ enteroendocrine progenitors (B), which in wild-type (WT) animals are located in the intestinal crypt compartment (A, arrows). Likewise, mutant animals are also devoid of chromogranin A+ (D), Cck/gastrin+ (F), Glp1+ (H), and Gip+ (J) cells, normally located in the villi of WT mice (arrows in C, E, G, and I), respectively. The age of the animals analyzed is 10-12 weeks. Original magnification, 10.

Copyright

This image is from Mellitzer G, J Clin Invest 2010 May 3;120(5):1708-21 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:161480

Associated Alleles

Symbol	Name
Neurog3^tm1Fgu	neurogenin 3; targeted mutation 1, Francois Guillemot
Neurog3^tm3.1Ggr	neurogenin 3; targeted mutation 3.1, Gerard Gradwohl
Tg(Vil1-cre)20Syr	transgene insertion 20, Sylvie Robine

Associated Genotypes

Allelic Composition	Genetic Background
Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL
Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL