Phenotype Image Detail

Image

Caption

Increased differentiation in the long bone in Flnb^tm1Vshn/Flnb^tm1Vshn (FlnB^-/-) mice. (A) Immunostaining of the long bones at P7 shows that the intensity of Sox9 is dramatically decreased in mutant mice, especially in the rapid proliferative zone and prehypertrophic zone. (B) Immunostaining for Runx2, a chondrocyte marker that is up-regulated during both endochondral and intramembranous ossification, is increased within chondrocyte progenitors within the proliferative zone, following Flnb knockout. For all experiments, n samples > or = 3 per variable, *= p< 0.05, **=p<0.01, ***= p <0.001 by t-test. Scale bars= 200 um.

Copyright

This image is from Hu J, PLoS One 2014;9(2):e89352, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:213355

Associated Alleles

Symbol	Name
Flnb^tm1Vshn	filamin, beta; targeted mutation 1, Volney Sheen

Associated Genotypes

Allelic Composition	Genetic Background
Flnb^tm1Vshn/Flnb^tm1Vshn	involves: 129S6/SvEvTac * C57BL/6