Phenotype Image Detail

Image

Caption

Yolk sac histology. (A) On day E8.5, Haematoxylin and Eosin (H&E) staining and alpha-SMA immunohistochemistry show comparable integrity, erythrocyte filling, alpha-SMA staining of the mesodermal layer of F3^tm1Fct/F3^tm1Fct (asTF) and wild-type (wt) yolk sacs. (B) On day E9.5, erythrocyte content, wall thickness, and number of alpha-SMA expressing cells are reduced in homozygous mutant yolk sacs. Mutant embryos are found at different stages of growth retardation and wasting. (C) On day E10.5, depletion of erythrocytes, reduced number of alpha-SMA+ cells, thinning and detachment of mesodermal from endodermal layer in homozygous mutant yolk sacs. No intact blood filled vessels can be detected anymore in homozygous mutant yolk sac en face preparations. (D) Quantification of erythrocyte and alpha-SMA+ cell number in E8.5-E10.5 yolk sacs of homozygous mutant and wt embryos ***p<0.001, **p<0.01.

Copyright

This image is from Sluka SH, PLoS One 2014;9(5):e97793, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:216519

Associated Alleles

Symbol	Name
F3^tm1Fct	coagulation factor III; targeted mutation 1, Felix C Tanner

Associated Genotypes

Allelic Composition	Genetic Background
F3^tm1Fct/F3^tm1Fct	involves: 129P2/OlaHsd * C57BL/6J