Phenotype Image Detail

Image

Caption

Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan Tg(T-cre)1Lwd/0 somites fail to organize the sclerotome and the peripheral nervous system (PNS). (A-L) H&E-stained sagittal sections (right is anterior in all images). (A) Vertical lines indicate somite boundaries of an E9.5 control embryo. (B) Dotted lines mark perturbations of somite boundaries in an E9.5 mutant embryo. dm, dermomyotome. (Magnification of 20 X in A and B.) (C) Magnified rectangular area of control (A). (D) Magnified rectangular area in mutant (B). (Magnification of 100 X in C and D.) Note greater myotomal mass (arrow) in control (C) than in mutant (D). (E and G) Sclerotomal condensations (brackets) form in the cervical and thoracic regions, respectively, in E11.5 control. (I and K) PNS organization of control is normal. (I) Dorsal root ganglia (drg) are segmented (lines), along with sclerotomal condensations (brackets). (K) Pedicle anlagen (marked as "P" with arrowheads) and spinal neurons (sn; arrows) display segmented organization. (F and H) The sclerotome is unsegmented (wide brackets) in the cervical and thoracic areas, respectively, in E11.5 mutant. (J and L) Defective PNS patterning in mutant. (J) Note drg are fused along the thoracic region. In addition, the sclerotome is unsegmented (bracket). (L) Note spinal nerves are disorganized (arrows). Pedicle primordia are indistinct and mixed with the myotome (asterisk). (Scale bars: 50 uM.)

Copyright

This image is from Lopez TP, Proc Natl Acad Sci U S A 2013 May 28;110(22):E2019-27. Copyright 2013 National Academy of Sciences, U.S.A. J:197427

Associated Alleles

Symbol	Name
Gt(ROSA)26Sor^tm1Fan	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Chen-Ming Fan
Tg(T-cre)1Lwd	transgene insertion 1, Mark Lewandoski

Associated Genotypes

Allelic Composition	Genetic Background
Gt(ROSA)26Sor^tm1Fan/Gt(ROSA)26Sor^tm1Fan Tg(T-cre)1Lwd/0	involves: C3H * C57BL/6 * C57BL/6J