Phenotype Image Detail

Image

Caption

Defects of arterial differentiation in Acvrl1 null embryos. (A) Whole-mount Pecam1 immunostaining shows defects of vascular remodeling in the embryo proper (b, d, and f) and yolk sac (h) in Acvrl1^tm1Dgen/Acvrl1^tm1Dgen (Acvrl1 KO) mice. Arrows indicate intersomitic vessels. Arrowheads indicate a well-branched vascular tree in the yolk sac, which is observed only in wild-type mice. a-f are shown with nuclear DAPI stain. (B and C) Expression of arterial endothelium marker genes, Efnb2 and Gja5/Cx40, diminishes in dorsal aorta of Acvrl1 null embryos (arrowheads in b and d) at E8.5 (B) and E9.5 (C). In contrast, the expression of Cdh5, which is detected both in the arteries and in the veins, is maintained (arrowheads in f). In situ hybridization is shown. (D) Expression of an endothelial marker, Pecam1, is maintained in dorsal aorta of an Acvrl1 null embryo (b) and in the yolk sac (d). Immunohistochemistry is shown. a-d are shown with nuclear DAPI stain. (E) Expression of Sm22/Tagln mRNA and Sm22 protein in the mural layers of dorsal aorta is significantly reduced in Acvrl1 null embryos (arrowheads). In situ hybridization (a and b) and immunohistochemistry (c and d) are shown. Insets in C and E show magnified views of dorsal aorta. c and d are shown with nuclear DAPI stain. (Scale bars: A-C and D, a and b, 50 um; and D, c and d, and E, 20 um.)

Copyright

This image is from Somekawa S, Proc Natl Acad Sci U S A 2012 Jul 24;109(30):12064-9. Copyright 2012 National Academy of Sciences, U.S.A. J:186473

Associated Alleles

Symbol	Name
Acvrl1^tm1Dgen	activin A receptor, type II-like 1; targeted mutation 1, Deltagen

Associated Genotypes

Allelic Composition	Genetic Background
Acvrl1^tm1Dgen/Acvrl1^tm1Dgen	involves: 129P2/OlaHsd * C57BL/6