Phenotype Image Detail

Image

Caption

Cc2d1a is important for neuronal survival and plasticity. A, Immunostaining and dendrite length measurements showing that total dendrite length per cell is lower in Cc2d1a^tm1Bisu/Cc2d1a^tm1Bisu (Mutant) neurons compared with wild-type (WT). Hippocampal neurons were cultured for 14 days, fixed, and stained with antibodies to MAP2. Dendrites were visualized by immunofluorescence microscopy. Fluorescent images were obtained and analyzed to measure total dendrite length per cell (p < 0.001). B, survival assay and immunostaining showing that the survival rate under oxidative stress is compromised in the mutant neurons. Hippocampal neurons were cultured for 36 hours, and stress was introduced by camptothecin (10 uM) or H202 (30 uM) treatment. Then the neurons were fixed and stained with Hoechst or DAPI to score healthy, intact nuclei as an estimate of cell survival. (p<0.002). Immunostaining and synapse number assay showed that the total synaptic number (C) and the synaptic density (D) is lower in the mutant neurons compared with WT neurons. Hippocampal neurons were cultured for 14 days, fixed, and stained with anti-MAP2 and anti-V GlutII antibodies. The number of synapses was counted per cell. Number of synapses per length of dendrite (100 um) was also calculated (p<0.002).

Copyright

This image is from Al-Tawashi A, J Biol Chem 2012 Apr 27;287(18):14644-58 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:184920

Associated Alleles

Symbol	Name
Cc2d1a^tm1Bisu	coiled-coil and C2 domain containing 1A; targeted mutation 1, Bing Su

Associated Genotypes

Allelic Composition	Genetic Background
Cc2d1a^tm1Bisu/Cc2d1a^tm1Bisu	Not Specified