Phenotype Image Detail

Image

Caption

Glycogen content in Gys2^tm1.1Pro/Gys2^tm1.1Pro (Conditional) and Gys2^tm1.1Pro/Gys2^tm1.1Pro Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 (LGSKO) mice. A and B, periodic acid-Schiff reagent staining of paraffin-embedded liver sections from random fed Gys2^tm1.1Pro/Gys2^tm1.1Pro (A) and Gys2^tm1.1Pro/Gys2^tm1.1Pro Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 (B) mice. The sections were counterstained for hematoxylin and eosin. All of the hepatocytes were stained pink in the liver sections from Conditional mice. Conversely, in LGSKO mouse sections only a small amount of cells (about 7%) were stained. The arrows point to those hepatocytes containing glycogen in the LGSKO mice sections. C and D, immunohistochemistry of paraffin-embedded liver sections of Conditional (C) and LGSKO (D) mice for CRE recombinase and counterstained with hematoxylin. Conditional mouse sections were negative for the staining, whereas LGSKO mouse sections were positively stained light brown in almost all the cells. However, some cells, marked by arrows, were not stained, indicating a lack of CRE recombinase protein.

Copyright

This image is from Irimia JM, J Biol Chem 2010 Apr 23;285(17):12851-61 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:162043

Associated Alleles

Symbol	Name
Gys2^tm1.1Pro	glycogen synthase 2; targeted mutation 1.1, Peter J Roach
Speer6-ps1^Tg(Alb-cre)21Mgn	spermatogenesis associated glutamate (E)-rich protein 6, pseudogene 1; transgene insertion 21, Mark A Magnuson

Associated Genotypes

Allelic Composition	Genetic Background
Gys2^tm1.1Pro/Gys2^tm1.1Pro Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * DBA