Phenotype Image Detail

Image

Caption

Decreased expression or cleavage of Sparcl1 (hevin) promotes gliosis in the mouse cerebellum. Immunohistochemical and H&E staining were performed on serially sectioned wild-type (WT) (A, D, and G), Sparcl1^tm1Pmc/Sparcl1^tm1Pmc (HN) (B, E, and H), or Adamts4^tm1Dqp/Adamts4^tm1Dqp (TS4N) (C, F, and I) mouse cerebellum. Areas of higher magnification stained with H&E (D-F) are indicated by boxes in A-C. In D-F, astrocyte/microglial cells are indicated by arrowheads. For immunohistochemistry (G-I), sections were co-stained with polyclonal goat anti-hevin (green) and polyclonal rabbit anti-GFAP antibodies (red). Arrows indicate GFAP⁺/hevin⁺ cells (short arrows) and areas of increased GFAP without hevin expression (long arrows). Images are representative of staining patterns seen in three independent experiments. WM, white matter; GL, granular layer. Scale bar, 40 um (A-C) and 10 um (D-I). J, quantification of total GFAP staining from a minimum of 6 fields per genotype is shown. Increased GFAP signal is evident in the white matter of Sparcl1^tm1Pmc/Sparcl1^tm1Pmc and Adamts4^tm1Dqp/Adamts4^tm1Dqp tissue, relative to WT brain (*, p<0.05).

Copyright

This image is from Weaver MS, J Biol Chem 2010 Feb 19;285(8):5868-77 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:159752

Associated Alleles

Symbol	Name
Adamts4^tm1Dqp	ADAM metallopeptidase with thrombospondin type 1 motif 4; targeted mutation 1, Duanqing Pei

Associated Genotypes

Allelic Composition	Genetic Background
Sparcl1^tm1Pmc/Sparcl1^tm1Pmc	involves: 129S1/Sv
Adamts4^tm1Dqp/Adamts4^tm1Dqp	involves: 129X1/SvJ * C57BL/6J