Phenotype Image Detail

Image

Caption

Npas4^tm1Ooe/Npas4^tm1Ooe brain shows increased vulnerability to a typical nerve-stress, glutamate excitotoxicity. 15-week-old Npas4^tm1Ooe/Npas4^tm1Ooe (NXF-KO) mice and wild-type (Wild) littermates were dosed with a glutamate analogue, kainate (28 mg/kg, intraperitoneal) (K1-8 for mutant, WK1-10 for Wild) or saline (S1-8 for KO, WS1-10 for Wild, only S1 and WS1 shown as representative). Microscopic images show glial fibrillary acidic protein (GFAP) immunoreactivity in the hippocampus (a part of CA1-2 and the DG region), one of the most sensitive sites for glutamate excitotoxicity. Three mutant mice (K6, -7, and -8) died by the fifth day after the kainate injection, whereas no control mice died.

Copyright

This image is from Ooe N, J Biol Chem 2009 Jan 9;284(2):1057-63 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:145569

Associated Alleles

Symbol	Name
Npas4^tm1Ooe	neuronal PAS domain protein 4; targeted mutation 1, Norihisa Ooe

Associated Genotypes

Allelic Composition	Genetic Background
Npas4^tm1Ooe/Npas4^tm1Ooe	involves: 129/Sv * C57BL/6