Phenotype Image Detail

Image

Caption

(A-C) Comparing E17.5 Porcn^tm1.1Lcm/Porcn⁺ Edil3^{Tg(Sox2-cre)1Amc}/0 (Porcn^delta/+) embryos with wild-type (WT) reveals a range of phenotypes that include cleft palate, tail hypoplasia, omphalocele, atrophic dermis through which the liver is visible, and tail/posterior axis truncation. cp, cleft palate; de, atrophic dermis; om, omphalocele; tr, tail/posterior axis truncation; tl, tail hypoplasia. (D-F) Alcian blue/alizarin red skeletal stains of WT and mutant forelimbs. The arrow in F indicates lack of autopod, accompanied by absence of ulna. (G-H) Alcian blue/nuclear fast red staining to reveal skeletal elements, including sternum, of E15.5 WT and mutant ventral body walls sectioned at the level of the heart. he, heart; st, sternum. (I and J) Immunostaining of sections semiadjacent to G and H (approximate positions indicated by red boxes) for E-cadherin (red) and the dermis/mesenchyme marker PDGF receptor-alpha (green). de, dermis; ep, epidermis. (Scale bars: G and H, 500 um; I and J, 50 um.)

Copyright

This image is from Barrott JJ, Proc Natl Acad Sci U S A 2011 Jul 18;():. Copyright 2011 National Academy of Sciences, U.S.A. J:173672

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Porcn^tm1.1Lcm	porcupine O-acyltransferase; targeted mutation 1.1, Lewis Murtaugh

Associated Genotypes

Allelic Composition	Genetic Background
Porcn^tm1.1Lcm/Porcn^+ Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA