Phenotype Image Detail

Image

Caption

Peroxisomal mosaicism-peroxisomal paucity, defective peroxisome proliferator-induced peroxisome proliferation, and spontaneous peroxisome proliferation in the liver of Acox1^tm1Jkr/Acox1^tm1Jkr mice. Light microscopic appearance of liver as revealed in semithin sections of tissue that was processed for the cytochemical localization of catalase using the alkaline 3', 3' -diaminobenzidine substrate. A, wild-type (+/+) mouse fed control diet shows peroxisomes randomly distributed in hepatocyte cytoplasm, as dark brown dots indicating the presence of diaminobenzidine reaction product (arrows); B and C, when these wild-type mice are treated with a peroxisome proliferator such as ciprofibrate, a marked proliferation of peroxisomes occurs in all hepatocytes (B), as evidenced by numerous brown-stained granules (C). D and E, Acox1^tm1Jkr/Acox1^tm1Jkr (-/-) mice (2 months old), show no catalase containing brown dots (peroxisomes) in a majority of hepatocytes except for a rare liver cell with clusters of peroxisomes appearing as brown granules (arrows in D, and E). F and G, these 2 month old mutant mice, male and female, show no peroxisome proliferation in a majority of hepatocytes when fed a diet containing a peroxisome proliferator, such as ciprofibrate. Fat droplets of different sizes are seen in a hepatocyte cytoplasm. Arrows point to an occasional hepatocyte with numerous peroxisomes but no steatosis (F and G). The sinusoidal erythrocytes are stained positively (dark brown color) due to the peroxidatic activity of hemoglobin. H and I, focal (H) and nodular proliferations (I) of hepatocytes occur in the livers of mutant mice, 5 months or older,and these hepatocytes reveal spontaneous peroxisome proliferation and are resistant to fatty change.

Copyright

This image is from Fan CY, J Biol Chem 1996 Oct 4;271(40):24698-710 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:35794

Associated Alleles

Symbol	Name
Acox1^tm1Jkr	acyl-Coenzyme A oxidase 1, palmitoyl; targeted mutation 1, Janardan K Reddy

Associated Genotypes

Allelic Composition	Genetic Background
Acox1^tm1Jkr/Acox1^tm1Jkr	involves: 129P2/OlaHsd * C57BL/6J