Phenotype Image Detail

Image

Caption

Morphogenesis of lung epithelium in Abca3^tm1Nina/Abca3^tm1Nina mice. A, a pair of newborn littermates, wild-type (+/+, right) and Abca3^tm1Nina/Abca3^tm1Nina (-/-, left). The isolated lung tissue in mutant mice was congested compared with that in wild-type mice. B, body weight and wet lung tissue weight of wild-type and mutant mice. Each open circle represents a single pup. The numbers in parentheses at the bottom represent total numbers of pups and wet lungs weighed. The bracketed numbers at top represents average weight. The body weight of mutant mice (n=22) was similar to that of wild-type mice (n=26) at birth. However, the wet lung weight of mutant mice (n=26) was significantly increased compared with that of wild-type mice (n=30). N.S., not significant; *, p<0.01. C-F, hematoxylin and eosin stained histological sections of the lung tissue in wild-type (C and E) and mutant (D and F) littermates. Severe atelectasis, pulmonary congestion, reduced septation, thickened mesenchyme, and columnar epithelium lining the peripheral saccules were observed in the mutant lung. Scale bars: 200 um in C and D; 50 um in E and F. G-I, consistent with the observed immaturity in the mutant lung, periodic acid-Schiff (PAS) staining demonstrated increased glycogen content in the bronchiolar epithelium of mutant mice (H and I) compared with control littermates (G). Scale bars: 50 um in G and H; 25 um in I.

Copyright

This image is from Ban N, J Biol Chem 2007 Mar 30;282(13):9628-34 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:121155

Associated Alleles

Symbol	Name
Abca3^tm1Nina	ATP-binding cassette, sub-family A member 3; targeted mutation 1, Nobuya Inagaki

Associated Genotypes

Allelic Composition	Genetic Background
Abca3^tm1Nina/Abca3^tm1Nina	involves: 129P2/OlaHsd * C57BL/6J