Phenotype Image Detail

Image

Caption

A: Newborn Prkaca^tm1Gsm/Prkaca⁺ Prkacb^tm1Gsm/Prkacb^tm1Gsm (PKA-deficient) mouse with spina bifida. The malformation in the spinal column in the mutant pup (right) is located at the thoracic and lumbar regions (arrow). The mouse at left is a normal wild-type (WT) littermate. B and C: Dorsal view of skeleton preparation from newborn mice. Bone is stained purple, and cartilage is stained blue. Mutant mouse (C) showing the opening of vertebral arches between forelimbs and hindlimbs (arrow) compared with wild-type (B). D and E: Neural tube defect (yellow arrows) at the region posterior to the forelimb buds of Prkaca^tm1Gsm/Prkaca⁺ Prkacb^tm1Gsm/Prkacb^tm1Gsm embryos (left in D) and Prkaca^tm1Gsm/Prkaca^tm1Gsm Prkacb^tm1Gsm/Prkacb⁺ (left in E) at E10.5. Approximately 25% of Prkaca^tm1Gsm/Prkaca^tm1Gsm Prkacb^tm1Gsm/Prkacb⁺ embryos develop both spinal neural tube defects (yellow arrow) together with exencephaly (red arrow) (left in E). Exencephaly is partially penetrant and only seen in embryos with a single functional Prkacb allele. Wild-type embryos are on the right (D and E).

Copyright

This image is from Huang Y, J Biol Chem 2002 May 31;277(22):19889-96 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:76765

Associated Alleles

Symbol	Name
Prkaca^tm1Gsm	protein kinase, cAMP dependent, catalytic, alpha; targeted mutation 1, G Stanley McKnight
Prkacb^tm1Gsm	protein kinase, cAMP dependent, catalytic, beta; targeted mutation 1, G Stanley McKnight

Associated Genotypes

Allelic Composition	Genetic Background
Prkaca^tm1Gsm/Prkaca^+ Prkacb^tm1Gsm/Prkacb^tm1Gsm	involves: 129X1/SvJ * C57BL/6
Prkaca^tm1Gsm/Prkaca^tm1Gsm Prkacb^tm1Gsm/Prkacb^+	involves: 129X1/SvJ * C57BL/6