Phenotype Image Detail

Image

Caption

Electron micrographs of yolk sac visceral endodermal cells from wild-type and Apob^tm3Sgy/Apob^tm3Sgy mice. Embryos were dissected in saline. Portions of the yolk sac membranes were removed for genotype analysis of Southern blotting, and the remainder were transferred to a fixation buffer. Sections were stained for lipids using imidazole-buffered osmium tetroxide. Sections from wild-type (A), Apob^tm3Sgy/Apob⁺ (+/-, B), and Apob^tm3Sgy/Apob^tm3Sgy (-/-, C) yolk sacs are shown. Intracellular cytosolic lipid droplets (LD) located at the basal aspect of the visceral endodermal cells are rarely observed in wild-type cells but are evident in increasing amounts in heterozygous and homozygous mutant cells. Arrows point to Golgi apparatus regions, which stain for lipids intensely in wild-type cells, much less intensely in heterozygous cells, and not at all in homozygous cells. Magnification, 5,600x.

Copyright

This image is from Farese RV Jr, J Lipid Res 1996 Feb;37(2):347-60 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text is from JLR. J:84977

Associated Alleles

Symbol	Name
Apob^tm3Sgy	apolipoprotein B; targeted mutation 3, Stephen G Young

Associated Genotypes

Allelic Composition	Genetic Background
Apob^tm3Sgy/Apob^+	involves: 129S7/SvEvBrd * C57BL/6
Apob^tm3Sgy/Apob^tm3Sgy	involves: 129S7/SvEvBrd * C57BL/6