Phenotype Image Detail

Image

Caption

Ultrastructure and basal intermediate filament proteins of esophagus of adult Krt14^tm1Efu/Krt14^tm1Efu mice. A and B: Esophageal tissues from 2 month old Krt14^tm1Efu/Krt14^tm1Efu and Krt14^tm1Efu/Krt14⁺ mice were fixed and processed for electron microscopy. Sections shown are from (a) mutant and (b) heterozygous mice. Note marked differences in the number and arrangement of keratin filaments (Kf) in these two samples. Nu, nuclei; Mi, mitochondria; arrowheads, basal lamina/hemidesmosomes. C: IF proteins were extracted from esophageal tissues of 2 day old and 2 month old wild-type mice. Proteins were resolved by SDS-polyacrylamide gel electrophoresis and subjected to immounoblot analysis and to identify keratin proteins. Note: Coomassie blue-stained gels of these samples revealed that the differences in absolute levels of K14 and K15 in these samples are similar to those that are reflected by alphaK14 and alphaK15 labeling. Bar, 0.45 um.

Copyright

This image is from Lloyd C, J Cell Biol 1995 Jun;129(5):1329-44, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:25763

Associated Alleles

Symbol	Name
Krt14^tm1Efu	keratin 14; targeted mutation 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Krt14^tm1Efu/Krt14^tm1Efu	involves: 129