Phenotype Image Detail

Image

Caption

Immunoelectron microscopy showing immunogold labeling for desmoplakin in intercalated discs of unfixed, freeze-sectioned myocardiac tissue of wild-type (a-c) and Jup^tm1Ruiz/Jup^tm1Ruiz mice (d-f). Note that in the wild-type mice, the desmosomes (D) display their typical ultrastructural organization and intense desmoplakin labeling. Higher resolution is presented in b, showing that the antibody-label is restricted to the plaque, whereas the membrane lipid bilayer structure (short arrows) and the mesoglea are free of label. By contrast, adherens junctions (arrows in c and insert) interspersed between the desmosomes, which anchor the bundles of sarcomeric microfilaments, are not significantly labeled. The triangle in (a) denotes one of the less frequent junctions in hearts of wild-type mice positive for desmoplakin but lacking typical desmosomal morphology. In the mutant embryos, the organization of the plaques in the intercalated disks is grossly altered. Here, extended junctional regions are frequent, which are almost entirely covered by a dense cytoplasmic plaque intensely labeled for desmoplakin (d). In these mutants, desmoplakin-positive plaques are also seen at junctions with fascia adherens-like morphology that anchor sarcomeric myofilament bundles (in the left and in the right of e), as well as on tiny junctions not yet classified (arrows in e and f). Bars: (b, insert in c) 0.2 um; (a, c-f) 0.5 um.

Copyright

This image is from Ruiz P, J Cell Biol 1996 Oct;135(1):215-25, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:64280

Associated Alleles

Symbol	Name
Jup^tm1Ruiz	junction plakoglobin; targeted mutation 1, Patricia Ruiz

Associated Genotypes

Allelic Composition	Genetic Background
Jup^tm1Ruiz/Jup^tm1Ruiz	involves: 129P2/OlaHsd * C57BL/6