Phenotype Image Detail

Image

Caption

A-H: Visualization of microvessels in E12 hindbrains using maximal intensity projections of z-stacked confocal images. Endothelial cells were stained with antibodies to CD31 (green); pericytes were stained with antibodies to the proteoglycan NG2 (red); and basement membrane was stained with antibodies to laminin (red). Wild-type (T-syn^+/+) embryos form a network of capillaries with uniform diameters and a regular branching pattern (A,C,E and G), whereas C1galt1^tm1Rpmc/C1galt1^tm1Rpmc (T-syn^-/-) embryos form capillaries with heterogeneous diameters and excessive, irregular branches (B,D,F and H). Insets in E and F are thin (5 um) optical slices of representative vessels enlarged 2.5-fold, which illustrate the abluminal relationship of the NG2-positive pericytes to the CD31-positive endothelial cells. P, perineural vascular plexus; V, ventricle. I and J: The architecture of large vessels (arrowheads) is similar in wild-type and mutant E12 yolk sacs, except that less blood fills the mutant vessels because of anemia. K and L: Staining of whole mounts of E12 yolk sacs with antibodies to CD31. More capillary branching and irregular vessel diameters are present in the mutant yolk sac. Arrowheads indicate narrowed capillaries at branch points, which suggests incomplete vascular pruning. Bars, 50 um.

Copyright

This image is from Xia L, J Cell Biol 2004 Feb 2;164(3):451-9, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:96775

Associated Alleles

Symbol	Name
C1galt1^tm1Rpmc	core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1; targeted mutation 1, Roger P McEver

Associated Genotypes

Allelic Composition	Genetic Background
C1galt1^tm1Rpmc/C1galt1^tm1Rpmc	involves: 129S1/Sv * C57BL/6J