Phenotype Image Detail

Image

Caption

A: Immunohistochemical identification of spermatogonia and spermatids using Hsp90alpha- and Trp2-specific antibodies, respectively, illustrates the aberrant location of these cells in testis of adult Pafah1b1^{Gt(IRESBetageo)1Hha}/Pafah1b1^{Gt(IRESBetageo)1Hha} mice (panels b and d) as compared to wild-type (panels a and c). The higher power magnification shows the elongated shape of wild-type spermatids (e) and mostly round spermatids in mutant testis (f). B: Hematoxylin and eosin (HE) staining and Tunel assay on sections of adult wild-type and mutant testis (a). Note the disrupted tubular structure, dilated vacuoles, and drastic reduction of spermatogenic cells in the mutant. A typical example of TUNEL labeling (brown cells) illustrates increased apoptosis of presumably spermatocytes in mutant testis. Statistical evaluation of apoptotic cells was performed by counting TUNEL-positive cells in at least 10 independent microscopic fields for each wild-type and mutant (b). The mean value indicates significantly elevated numbers of apoptotic cells in the mutant.

Copyright

This image is from Nayernia K, J Biol Chem 2003 Nov 28;278(48):48377-85 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:86720

Associated Alleles

Symbol	Name
Pafah1b1^Gt(IRESBetageo)1Hha	platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; gene trap 1, Hans-Henning Arnold

Associated Genotypes

Allelic Composition	Genetic Background
Pafah1b1^Gt(IRESBetageo)1Hha/Pafah1b1^Gt(IRESBetageo)1Hha	involves: 129/Sv * NMRI