Phenotype Image Detail

Image

Caption

A-B: Foxm1^tm1.1Rhc/Foxm1^tm1.1Rhc (Foxm1-/-) 15.5 dpc embryos display reduced Pecam-1 staining. Embryonic lungs from wild type (WT, A) and mutant (B) 17.5 dpc embryos were fixed, paraffin-embedded, sectioned and then immunohistochemically stained with Pecam-1 antibody followed by biotinylated secondary antibody, avidin-alkaline phosphatase complex, and BCIP/NBT substrate. Lung sections were counterstained with nuclear fast red. C-D: Foxm1 deficiency does not cause an increase in apoptosis. To detect apoptotic cells, paraffin lung sections were prepared from wild type and mutant 17.5 dpc embryos and used for TUNEL assay. E-F: Mutant lungs displayed a reduced number of pulmonary blood vessels. Hematoxylin and eosin and Pecam-1 stained lung sections from 15.5 and 17.5 dpc mutant and wild type embryos were used to count the number of pulmonary vessels in x400 microscope field. Five random lung sections were counted in four different mouse lungs to calculate the mean number of large vessels +/- S.D. A p value less than or equal to 0.05 is shown with an asterisk. G: Mutant lungs exhibit diminished Flk1 expression. Total lung RNA was prepared from WT and mutant 17.5 dpc mouse embryos and used to analyze for Flk1 and cyclophilin mRNA levels by RNase protection assay. Abbreviations: Br, bronchi; Ar, artery. Magnification: A-D, x200.

Copyright

This image is from Kim IM, J Biol Chem 2005 Jun 10;280(23):22278-86 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:100263

Associated Alleles

Symbol	Name
Foxm1^tm1.1Rhc	forkhead box M1; targeted mutation 1.1, Robert H Costa

Associated Genotypes

Allelic Composition	Genetic Background
Foxm1^tm1.1Rhc/Foxm1^tm1.1Rhc	involves: 129X1/SvJ * C57BL/6