Phenotype Image Detail

Image

Caption

Histological analysis of Bmpr1a^tm2.1Bhr/Bmpr1a^tm2.1Bhr Bmpr1b^tm1Kml/Bmpr1b^tm1Kml Tg(Col2a1-cre)1Bhr/? mice (Bmpr1a^CKO; Bmpr1b^-/-). A and B: Alcian blue staining of cartilage condensations in E12.5 limb buds. Images in B are magnifications of the boxed region in A. In the wild type (WT) limb, cells in the most proximal element have differentiated into overt chondrocytes; cells in the distal elements are prechondrocytic. In the mutant limb, all condensations are delayed in a prechondrocytic state. C: Immunohistochemistry (IHC) of BMPR1A at E12.5 demonstrating that BMPR1A is not expressed in mutant condensations. D: Alcian blue staining of E13.5 limbs. Condensations in mutant limbs are reduced and remain in a prechondrocytic state. E: Magnification of boxed areas in D. In contrast to the fully differentiated wild type digits, shells of prechondrocytes surround cells that do not stain for alcian blue in mutants. F: IHC of type 2 collagen (brown) counterstained with hematoxylin (blue) of E14.5 digits. A dense perichondrium is present, but core cells do not differentiate into chondrocytes. G and H: Alcian blue staining of thoracic vertebral bodies in E13.5 (G) and E14.5 (H) littermates. I: Alican blue staining of neural crest-derived nasal cavity at E14.5 embryos. p, proximal; d, distal; u, ulna; r, radius; n, notochord; vb, vertebral body; np, nucleus pulposi.

Copyright

This image is from Yoon BS, Proc Natl Acad Sci U S A 2005 Apr 5;102(14):5062-7. Copyright 2005 National Academy of Sciences, U.S.A. J:97410

Associated Alleles

Symbol	Name
Bmpr1a^tm2.1Bhr	bone morphogenetic protein receptor, type 1A; targeted mutation 2.1, Richard R Behringer
Bmpr1b^tm1Kml	bone morphogenetic protein receptor, type 1B; targeted mutation 1, Karen M Lyons
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
Bmpr1a^tm2.1Bhr/Bmpr1a^tm2.1Bhr Bmpr1b^tm1Kml/Bmpr1b^tm1Kml Tg(Col2a1-cre)1Bhr/?	involves: 129S/SvEv * 129S7/SvEvBrd * C57BL/6 * SJL