Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Alox5. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Alox5
  • participates in the following biological processes:
    • leukotriene biosynthetic process ^[4]
  • is located in the following cellular components:
    • cytoplasm ^[3]
    • nucleus ^[3]
• The gene product of Alox5 has been shown to bind to the gene products of Alox5ap. ^[4]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Alox5
  • participates in the following biological processes:
    • inflammatory response ^[5]
    • leukotriene biosynthetic process ^[2]
    • leukotriene metabolic process ^[5]
    • leukotriene production involved in inflammatory response ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Alox5:
  • participates in the following biological processes:
    • acute inflammatory response
    • arachidonic acid metabolic process
    • leukotriene biosynthetic process
    • lipoxygenase pathway
    • oxidation-reduction process
    • positive regulation of vasoconstriction
    • sensory perception of pain
  • performs the following molecular functions:
    • arachidonate 5-lipoxygenase activity
    • iron ion binding
  • is located in the following cellular components:
    • cytosol
    • dendrite
    • nuclear envelope
    • nuclear envelope lumen
    • nuclear matrix
    • nuclear membrane
    • nucleus
    • sarcolemma

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Summary text based on GO annotations supported by structural data

• Alox5 encodes a protein or proteins that contain the following InterPro domains: Lipase/lipooxygenase, PLAT/LH2, Lipoxygenase, Lipoxygenase, C-terminal, Lipoxygenase, conserved site, Lipoxygenase, iron binding site, Lipoxygenase, LH2, Lipoxygenase, mammalian, indicating that the gene product:
  • performs the following molecular functions:
    • lipoxygenase activity
    • metal ion binding
    • oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Alox5 indicates that it:
  • performs the following molecular functions:
    • metal ion binding
    • oxidoreductase activity
    • oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
  • is located in the following cellular components:
    • membrane

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References

1. Byrum RS et al. (1997) Role of the 5-lipoxygenase-activating protein (FLAP) in murine acute inflammatory responses. J Exp Med, 185:1065-75. (PubMed:9091580)
2. Edmiston JS et al. (1997) A transforming growth factor-beta regulable RNA-binding protein interacts specifically with germline Ig alpha transcripts. Int Immunol, 9:427-33. (PubMed:9088981)
3. Jones SM et al. (2002) Structural and functional criteria reveal a new nuclear import sequence on the 5-lipoxygenase protein. J Biol Chem, 277:38550-6. (PubMed:12140292)
4. Mandal AK et al. (2008) The nuclear membrane organization of leukotriene synthesis. Proc Natl Acad Sci U S A, 105:20434-9. (PubMed:19075240)
5. Segal BH et al. (2002) Thioglycollate peritonitis in mice lacking C5, 5-lipoxygenase, or p47(phox): complement, leukotrienes, and reactive oxidants in acute inflammation. J Leukoc Biol, 71:410-6. (PubMed:11867678)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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