Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Add2. (This text reflects annotations as of Wednesday, January 23, 2013.)

---

Summary from NCBI RefSeq

This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012]

---

Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Add2
  • performs the following molecular functions:
    • structural molecule activity ^[1]
  • is located in the following cellular components:
    • membrane ^[1]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Add2
  • participates in the following biological processes:
    • hemopoiesis ^[1]

---

Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Add2:
  • participates in the following biological processes:
    • actin filament bundle assembly
    • barbed-end actin filament capping
    • ion transport
    • positive regulation of protein binding
  • performs the following molecular functions:
    • actin filament binding
    • protein heterodimerization activity
    • protein homodimerization activity
    • spectrin binding
  • is located in the following cellular components:
    • F-actin capping protein complex

---

Summary text based on GO annotations supported by structural data

• Add2 encodes a protein or proteins that contain the following InterPro domains: Class II aldolase/adducin N-terminal, indicating that the gene product:
  • performs the following molecular functions:
    • metal ion binding

---

Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Add2 indicates that it:
  • performs the following molecular functions:
    • actin binding
    • calmodulin binding
  • is located in the following cellular components:
    • cytoplasm
    • cytoskeleton
    • plasma membrane

---

References

1. Gilligan DM et al. (1999) Targeted disruption of the beta adducin gene (Add2) causes red blood cell spherocytosis in mice. Proc Natl Acad Sci U S A, 96:10717-22. (PubMed:10485892)

---

---

Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

---