Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Rspo1. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Rspo1
  • participates in the following biological processes:
    • positive regulation of canonical Wnt signaling pathway ^[1]
  • performs the following molecular functions:
    • heparin binding ^[4]
  • is located in the following cellular components:
    • extracellular space ^{[3, 4]}
    • nucleus ^[3]
• The gene product of Rspo1 has been shown to bind to the gene products of Lgr4. ^[1] Researchers have inferred, based on physical interactions, that the gene product of Rspo1
  • participates in the following biological processes:
    • canonical Wnt signaling pathway ^[4]
  • performs the following molecular functions:
    • receptor binding ^[4]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Rspo1
  • participates in the following biological processes:
    • male meiosis ^[2]
    • positive regulation of non-canonical Wnt signaling pathway ^[2]
    • regulation of gene expression ^[2]
    • regulation of male germ cell proliferation ^[2]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Rspo1:
  • participates in the following biological processes:
    • positive regulation of canonical Wnt signaling pathway
    • positive regulation of protein phosphorylation
    • positive regulation of Wnt signaling pathway
    • regulation of receptor internalization
  • performs the following molecular functions:
    • G-protein coupled receptor binding
    • receptor binding

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Rspo1 indicates that it:
  • participates in the following biological processes:
    • response to stimulus
    • Wnt signaling pathway
  • is located in the following cellular components:
    • extracellular region

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References

1. Carmon KS et al. (2011) R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling. Proc Natl Acad Sci U S A, 108:11452-7. (PubMed:21693646)
2. Chassot AA et al. (2011) RSPO1/beta-catenin signaling pathway regulates oogonia differentiation and entry into meiosis in the mouse fetal ovary. PLoS One, 6:e25641. (PubMed:21991325)
3. Kamata T et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants Biochim Biophys Acta, 1676:51-62. (PubMed:14732490)
4. Nam JS et al. (2006) Mouse cristin/R-spondin family proteins are novel ligands for the Frizzled 8 and LRP6 receptors and activate beta-catenin-dependent gene expression. J Biol Chem, 281:13247-57. (PubMed:16543246)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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