Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Prdm16. (This text reflects annotations as of Wednesday, January 23, 2013.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of August 23, 2007. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Prdm16
  • participates in the following biological processes:
    • brown fat cell differentiation ^{[3, 4, 7]}
    • negative regulation of transcription, DNA-dependent ^[4]
    • neurogenesis ^[5]
    • positive regulation of transcription, DNA-dependent ^{[3, 4]}
    • regulation of cellular respiration ^{[4, 6]}
    • somatic stem cell maintenance ^[2]
    • white fat cell differentiation ^[4]
  • performs the following molecular functions:
    • sequence-specific DNA binding ^[6]
    • transcription coactivator activity ^{[6, 7]}
  • is located in the following cellular components:
    • nucleus ^[4]
    • transcriptional repressor complex ^[4]
• The gene product of Prdm16 has been shown to bind to the gene products of Cebpa, Cebpb, Cebpd, Ppara, Pparg, Ppargc1a, Smad3. ^{[3, 4, 6, 7, 8]} Researchers have inferred, based on physical interactions, that the gene product of Prdm16
  • performs the following molecular functions:
    • SMAD binding ^[8]
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Prdm16
  • participates in the following biological processes:
    • brown fat cell differentiation ^[6]
    • negative regulation of transforming growth factor beta receptor signaling pathway ^[1]
    • palate development ^[1]
    • tongue development ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Prdm16:
  • participates in the following biological processes:
    • negative regulation of granulocyte differentiation
    • negative regulation of transcription from RNA polymerase II promoter
    • negative regulation of transcription, DNA-dependent
    • negative regulation of transforming growth factor beta receptor signaling pathway
    • positive regulation of transcription, DNA-dependent
  • performs the following molecular functions:
    • sequence-specific DNA binding
  • is located in the following cellular components:
    • nucleus

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Summary text based on GO annotations supported by structural data

• Prdm16 encodes a protein or proteins that contain the following InterPro domains: SET domain, Zinc finger C2H2-type/integrase DNA-binding domain, Zinc finger, C2H2, Zinc finger, C2H2-like, indicating that the gene product:
  • performs the following molecular functions:
    • nucleic acid binding
    • zinc ion binding
  • is located in the following cellular components:
    • intracellular

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Prdm16 indicates that it:
  • participates in the following biological processes:
    • cell differentiation
    • regulation of transcription, DNA-dependent
    • transcription, DNA-dependent
  • performs the following molecular functions:
    • DNA binding
    • metal ion binding

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References

1. Bjork BC et al. (2010) Prdm16 is required for normal palatogenesis in mice. Hum Mol Genet, 19:774-89. (PubMed:20007998)
2. Deneault E et al. (2009) A functional screen to identify novel effectors of hematopoietic stem cell activity. Cell, 137:369-79. (PubMed:19379700)
3. Kajimura S et al. (2009) Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex. Nature, 460:1154-8. (PubMed:19641492)
4. Kajimura S et al. (2008) Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex. Genes Dev, 22:1397-409. (PubMed:18483224)
5. Kinameri E et al. (2008) Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis. PLoS ONE, 3:e3859. (PubMed:19050759)
6. Seale P et al. (2007) Transcriptional control of brown fat determination by PRDM16. Cell Metab, 6:38-54. (PubMed:17618855)
7. Seale P et al. (2008) PRDM16 controls a brown fat/skeletal muscle switch. Nature, 454:961-7. (PubMed:18719582)
8. Warner DR et al. (2007) PRDM16/MEL1: a novel Smad binding protein expressed in murine embryonic orofacial tissue. Biochim Biophys Acta, 1773:814-20. (PubMed:17467076)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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