Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Ehhadh. (This text reflects annotations as of Thursday, July 24, 2014.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Ehhadh
  • is located in the following cellular components:
    • mitochondrion ^{[2, 3]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Ehhadh
  • participates in the following biological processes:
    • fatty acid beta-oxidation ^[1]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Ehhadh:
  • participates in the following biological processes:
    • internal protein amino acid acetylation
    • oxidation-reduction process
  • performs the following molecular functions:
    • 3-hydroxyacyl-CoA dehydrogenase activity
    • enzyme binding
    • receptor binding
  • is located in the following cellular components:
    • cytoplasm
    • cytosol
    • intracellular membrane-bounded organelle
    • nucleus
    • peroxisome

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Summary text based on GO annotations supported by structural data

• Ehhadh encodes a protein or proteins that contain the following InterPro domains: 3-hydroxyacyl-CoA dehydrogenase, C-terminal, 3-hydroxyacyl-CoA dehydrogenase, conserved site, 3-hydroxyacyl-CoA dehydrogenase, NAD binding, 6-phosphogluconate dehydrogenase, C-terminal-like, Crotonase superfamily, Dehydrogenase, multihelical, Enoyl-CoA hydratase/isomerase, conserved site, NAD(P)-binding domain, indicating that the gene product:
  • participates in the following biological processes:
    • fatty acid metabolic process
    • metabolic process
  • performs the following molecular functions:
    • catalytic activity
    • coenzyme binding
    • oxidoreductase activity
    • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Ehhadh indicates that it:
  • participates in the following biological processes:
    • fatty acid metabolic process
    • lipid metabolic process
    • metabolic process
  • performs the following molecular functions:
    • catalytic activity
    • isomerase activity
    • lyase activity
    • oxidoreductase activity
• Ehhadh has been associated with the Enzyme Commission numbers: 1.1.1.35, 4.2.1.17, 5.3.3.8, which implies that the gene product:
  • performs the following molecular functions:
    • dodecenoyl-CoA delta-isomerase activity
    • enoyl-CoA hydratase activity

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References

1. Dirkx R et al. (2007) Beta-oxidation in hepatocyte cultures from mice with peroxisomal gene knockouts. Biochem Biophys Res Commun, 357:718-23. (PubMed:17442273)
2. Mootha VK et al. (2003) Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell, 115:629-40. (PubMed:14651853)
3. Pagliarini DJ et al. (2008) A mitochondrial protein compendium elucidates complex I disease biology. Cell, 134:112-23. (PubMed:18614015)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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