| Gene: 1520 | Name: Adcy3 | Family: Cyclase | Subfamily: Adenylate | Accession: AF458089 | GI: 18092642 |
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Gene 1520
Summary of Phenotypic Analysis
Changes related to genotype:
· Lethality: Homozygous mice frequently do not survive to weaning age.
There were no significant differences detected in the homozygous or heterozygous mutant mice when compared with age- and gender-matched wild-type control mice.
Weaned progeny from the heterozygous matings were genotyped. No homozygous mutant mice were identified by PCR, whereas wild-type and heterozygous mutant mice were present. The genotypic ratio suggests either an embryonic, perinatal or juvenile lethal phenotype.
ES cells derived from the 129/OlaHsd mouse substrain were used to generate chimeric mice. F1 mice were generated by breeding with C57BL/6 females. F2 mutant mice were produced by intercrossing F1 heterozygous males and females.
Wild-type control mice, and homozygous and heterozygous mutant mice, were evaluated by the following examinations or tests:
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Keywords |
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developmental
lethality |
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perinatal
lethal |
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Gene
1520 RT-PCR Summary: LacZ Summary: Expression: Harderian Glands Aorta Heart Lung Kidney Urinary Bladder Salivary Glands Tongue Ear, external Male Reproductive Systems Penis Coagulating Gland Prostate and Ampullary Gland Female Reproductive Systems
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Gene
1520 There were no significant
differences detected in the homozygous or heterozygous mutant mice when
compared with age- and gender-matched wild-type control mice. The following mice were
necropsied. Body weight, body length, and organ weights were obtained, and
gross pathological findings were recorded. 20 Day Cohort Mouse ID numbers
are as follows: Necropsy Findings: There were no genotype-related or
biologically significant differences noted between mutant and wild-type
control mice for any of the parameters evaluated at necropsy. Incidental
lesions may have been present in some tissues. For example, in a 300 day
heterozygous mutant female (256704) increased subcutaneous fat correlated
with high body weight at necropsy and high fat % at densitometer examination.
These findings were considered to represent background lesions occasionally
seen in this strain of mice, lesions due to spontaneous disease, age-related
lesions, and/or lesions of a nonspecific etiology. They were not
considered to be related to genotype. Body and Organ Weight Findings: Body weight and several organ
weights, but not organ weight/body weight ratios, were high in a 300 day
heterozygous mutant female (256704). The first finding correlated with high
body weight in another 300 day heterozygous mutant female at aging metrics
examination. We have not reported these findings as phenotypic changes but we
have presented them here for your consideration. Other differences in body length,
body weight, organ weights, and/or organ weight to body weight ratios were
present between individual mice. For example, The variability between
mice usually fell within our historical reference ranges and was not
correlated with genotype. |
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Gene
1520 There were no significant differences detected in the homozygous or heterozygous mutant mice when compared with age- and gender-matched wild-type control mice. Tissues from the following mice were evaluated histologically. 20 Day Cohort Mouse ID numbers
are as follows: The following tissues were examined and considered to have no genotype-related abnormality: brain, pituitary gland, ears, nasal cavity, salivary glands, oral cavity, lymph nodes, aorta, lungs, liver, gallbladder, pancreas, spleen, kidneys, urinary bladder, stomach, small and large intestines, larynx, esophagus, trachea, thyroid gland, thymus gland, tongue, skeletal muscle, sciatic nerve, mammary glands, vertebrae, spinal cord, bone (skull, sternum, femur, tibia, and stifle joint), reproductive tract (including gonads), eyes, Harderian glands, integumentary system (skin and either clitoral or preputial glands), and bone marrow. Bone marrow was examined in sections of sternum, vertebrae, and/or femur and tibia. Marrow cellularity, myeloid:erythroid (M:E) ratio, myeloid and erythroid maturation sequences, and numbers of megakaryocytes were evaluated. Incidental lesions were present in some tissues. These findings were considered to represent background lesions occasionally seen in this strain of mice, lesions due to spontaneous disease, age-related lesions, and/or lesions of a nonspecific etiology. They were not considered to be genotype related. |
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Gene
1520 There were no significant
differences in the heterozygous mutant mice when compared with age- and
gender-matched wild-type control mice. Blood samples from the following
mice were evaluated by a complete blood count and differential cell count. 49 Day Cohort Mouse ID numbers
are as follows: |
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Gene
1520 There were no significant
differences in the heterozygous mutant mice when compared with age- and
gender-matched wild-type control mice. Serum samples from the following
mice were evaluated by a clinical chemistry panel. 49 Day Cohort Mouse ID numbers
are as follows: |
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Gene
1520 There were no significant differences detected in the homozygous or heterozygous mutant mice when compared with age- and gender-matched wild-type control mice. The following mice were evaluated by dual-energy x-ray absorptiometry. 49 Day Cohort Mouse ID numbers
are as follows: Densitometric Findings: In a 300 day heterozygous mutant
female (256704) high fat % and total tissue mass correlated with increased
subcutaneous fat and high body weight at necropsy examination, and with high
body weight in another 300 day heterozygous mutant female at aging metrics
examination. We have not reported these findings as phenotypic changes
but we have presented them here for your consideration. Other incidental densitometric
differences may have been present between some mice. These findings were
considered to represent background differences occasionally seen in this
strain of mice, differences due to spontaneous disease, age-related changes,
differences due to procedural artifacts, and/or differences of a nonspecific
etiology. They were not considered to be genotype related. |
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Gene
1520 There were no significant
differences detected in the homozygous or heterozygous mutant mice when
compared with age- and gender-matched wild-type control mice. The following mice were evaluated
by physical examination. 20 Day Cohort Mouse ID numbers
are as follows: Individual homozygous mutant and
heterozygous mutant mice had only occasional minor differences in observed
physical features compared to wild-type control mice. These findings were
considered to represent individual variability, background features
occasionally seen in this strain of mice, findings due to spontaneous
disease, age-related findings, and/or findings of a nonspecific etiology.
However, none of these differences was regarded as biologically significant
or genotype related. |
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Gene
1520 There were no significant
differences detected in the heterozygous mutant mice when compared with age-
and gender-matched wild-type control mice. Body weights and body lengths
were measured for mice at 49, 90, 180, and 300 days of age. 49 Day Cohort Mouse ID numbers
are as follows: Body weight and/or body
weight/body length ratio were high compared to historical but not
contemporaneous wild-type control mice in three 90 day heterozygous
mutant females (256704, 256705, 256708), one 90 day heterozygous mutant male
(256766), one 300 day heterozygous mutant female (256708) and one 300 day
heterozygous mutant male (256766). These findings correlated with increased
subcutaneous fat and high body weight at necropsy examination and high fat %
and total tissue mass at densitometer examination in another 300 day
heterozygous mutant female. We have not reported these findings as phenotypic
changes but we have presented them here for your consideration. Other differences in body length and body weight were present between individual mice. The variability between mice usually fell within our historical reference ranges and was not correlated with genotype. |
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Gene
1520 Heterozygous mutant and wild-type
control mice were evaluated for phenotypic changes by testing on seven
behavioral tasks: Open field test, Tail suspension test, Rotarod test,
Startle test, Tail flick test, Hot plate test, and Metrazol test. Mouse ID numbers are as follows
for the N1 generation: Behavior Findings: |