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Gene Expression Data
Assay Details
Assay
Reference: J:116229 Roulet M, et al., A comprehensive study of the spatial and temporal expression of the col5a1 gene in mouse embryos: a clue for understanding collagen V function in developing connective tissues. Cell Tissue Res. 2007 Feb;327(2):323-32
Assay type: RNA in situ
MGI Accession ID: MGI:6278131
Gene symbol: Col5a1
Gene name: collagen, type V, alpha 1
Probe: Col5a1 probe2
Probe preparation: Antisense RNA labelled with S35
Visualized with: Autoradiography
Results
Specimen E7.5 not shown: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo Absent

Specimen 1A/B embryo: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: dorsal aorta Present Regionally restricted 1A/B Expression first detectable at 8.5 dpc, restricted to wall of dorsal aorta.

Specimen 1A/B maternal: postnatal adult (more )
Structure Level Pattern Image Note
TS28: uterus Weak 1A/B Authors report expression in the adnexa. (Authors state a longer exposure was necessary to detect expression.)

Specimen 1C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: blood vessel Strong 1C Expression in a broad variety of tissues with especially high levels of expression in nervous tissue and blood vessels.
TS17: nervous system Strong 1C Expression in a broad variety of tissues with especially high levels of expression in nervous tissue and blood vessels.
TS17: neural tube Present Regionally restricted 1C Expression in the neuroepithelium.
TS17: primitive ventricle Present Regionally restricted 1C Expression in the trabeculated wall of the common ventricular chamber of the heart.
TS17: branchial arch Present 1C
TS17: dorsal aorta Present 1C
TS17: mesonephric tubule Present 1C
TS17: embryo mesenchyme Present Regionally restricted 1C Authors report expression in some areas of mesenchyme.

Specimen 1E: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: head mesenchyme Present 1E Expression more restricted to developing mesenchyme, especially to cephalic mesenchyme.

Specimen 1F: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: head mesenchyme Present Regionally restricted 1F Expression in brain mesenchymal cells. Expression in cranial bone tissue (calvaria).

Specimen 1G: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: trunk paraxial mesenchyme Present Regionally restricted 1G Expression in trunk region, around somites.

Specimen 2A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: head mesenchyme Weak 2A Authors state expression is low compared with that of later stages. Expression was less diffuse and more confined to regions such as craniofacial tissues and vertebral column.
TS20: vertebral pre-cartilage condensation Weak 2A Authors state expression is low compared with that of later stages. Expression was less diffuse and more confined to regions such as craniofacial tissues and vertebral column.

Specimen 2B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: dermis Strong 2B Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
TS24: head mesenchyme Strong 2B Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
TS24: intestine mesenchyme Strong 2B Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.

Specimen 2D: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: dermis Strong 2D Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
TS26: head mesenchyme Strong 2D Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
TS26: intestine mesenchyme Strong 2D Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.

Specimen 3A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: surface ectoderm Present Regionally restricted 3A Expression in undifferentiated flattened cells of the surface ectoderm and in some underlying cells.

Specimen 3B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: epidermis Absent 3B No expression when epithelial cells become cuboidal.

Specimen 3C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: dermis Present 3C Expression strictly confined to underlying dermal cells.

Specimen 3D: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: dermis Present Regionally restricted 3D More intense expression in the papillary layer, the upper layer of dermis.

Specimen 3E: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: dermis Weak 3E Lower intensity of expression compared with previous stages.

Specimen 3G: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: vibrissa follicle Present Diffuse 3G Authors state expression around vibrissa follicle was more uniform and diffuse than that of Col1a1.

Specimen 4A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: vertebral pre-cartilage condensation Strong Regionally restricted 4A High levels of expression between the undifferentiated vertebral bodies.

Specimen 4B: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: rib cartilage condensation Present Regionally restricted 4B Expression in perichondrium surounding cartilaginous ribs.

Specimen 4C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: rib cartilage condensation Present Regionally restricted 4C Expression restricted to perichondrium surrounding cartilaginous ribs and in neighboring mesenchymal cells. No expression in chondrocytes.

Specimen 4E/F: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: vertebra Strong Regionally restricted 4E/F High expression in fibroblasts of periosteum and osteoblasts of ossified vertebrae.

Specimen 4G: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: mandible Strong Regionally restricted 4G Particularly strong expression in periosteum and in osteoblasts of mandibular arches.

Specimen 4H/I: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: incisor dental papilla Strong Regionally restricted 4H/I High level of expression in developing teeth, particulary in odontoblasts. Expression also detected in pulp cells.

Specimen 6A/B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: connective tissue Present Regionally restricted 6A/B Authors report expression in developing ligaments and tendons.

Specimen 6C/D: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: umbilical cord Present Regionally restricted 6C/D Authors report robust expression in vessel walls of the umbilical cord.

Specimen 6E: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: submandibular gland mesenchyme Present 6E Expression in mesenchyme surrounding acini of the submandibular salivary gland.

Specimen 6F: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine mesenchyme Present 6F

Specimen 6G/H: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: intestine mesenchyme Present 6G/H


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/19/2021
MGI 6.17
The Jackson Laboratory