Gene Expression Data
Assay Details
Assay
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Reference:
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J:116229
Roulet M, et al., A comprehensive study of the spatial and temporal expression of the col5a1 gene in mouse embryos: a clue for understanding collagen V function in developing connective tissues. Cell Tissue Res. 2007 Feb;327(2):323-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6278131
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Gene symbol:
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Col5a1
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Gene name:
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collagen, type V, alpha 1
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Probe:
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Col5a1 probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
E7.5 not shown:
embryonic day 7.5
(more )
Specimen
E7.5 not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 7.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
1A/B embryo:
embryonic day 8.5
(more )
Specimen
1A/B embryo:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS13: dorsal aorta |
Present |
Regionally restricted |
1A/B
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Expression first detectable at 8.5 dpc, restricted to wall of dorsal aorta.
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Specimen
1A/B maternal:
postnatal adult
(more )
Specimen
1A/B maternal:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: uterus |
Weak |
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1A/B
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Authors report expression in the adnexa. (Authors state a longer exposure was necessary to detect expression.)
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Specimen
1C:
embryonic day 10.5
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
1E:
embryonic day 11.5
(more )
Specimen
1E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: head mesenchyme |
Present |
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1E
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Expression more restricted to developing mesenchyme, especially to cephalic mesenchyme.
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Specimen
1F:
embryonic day 11.5
(more )
Specimen
1F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: head mesenchyme |
Present |
Regionally restricted |
1F
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Expression in brain mesenchymal cells. Expression in cranial bone tissue (calvaria).
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Specimen
1G:
embryonic day 11.5
(more )
Specimen
1G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
2A:
embryonic day 12.5
(more )
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: head mesenchyme |
Weak |
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2A
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Authors state expression is low compared with that of later stages. Expression was less diffuse and more confined to regions such as craniofacial tissues and vertebral column.
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TS20: vertebral pre-cartilage condensation |
Weak |
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2A
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Authors state expression is low compared with that of later stages. Expression was less diffuse and more confined to regions such as craniofacial tissues and vertebral column.
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Specimen
2B:
embryonic day 16.5
(more )
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: dermis |
Strong |
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2B
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Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
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TS24: head mesenchyme |
Strong |
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2B
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Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
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TS24: intestine mesenchyme |
Strong |
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2B
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Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
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Specimen
2D:
embryonic day 18.5
(more )
Specimen
2D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: dermis |
Strong |
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2D
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Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
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TS26: head mesenchyme |
Strong |
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2D
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Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
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TS26: intestine mesenchyme |
Strong |
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2D
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Authors report intense expression in craniofacial tissues, dermis, intestinal mesenchyme, and other tissues.
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Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: surface ectoderm |
Present |
Regionally restricted |
3A
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Expression in undifferentiated flattened cells of the surface ectoderm and in some underlying cells.
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Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: epidermis |
Absent |
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3B
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No expression when epithelial cells become cuboidal.
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Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: dermis |
Present |
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3C
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Expression strictly confined to underlying dermal cells.
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Specimen
3D:
embryonic day 18.5
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: dermis |
Present |
Regionally restricted |
3D
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More intense expression in the papillary layer, the upper layer of dermis.
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Specimen
3E:
postnatal newborn
(more )
Specimen
3E:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS27: dermis |
Weak |
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3E
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Lower intensity of expression compared with previous stages.
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Specimen
3G:
embryonic day 18.5
(more )
Specimen
3G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: vibrissa follicle |
Present |
Diffuse |
3G
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Authors state expression around vibrissa follicle was more uniform and diffuse than that of Col1a1.
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Specimen
4A:
embryonic day 12.5
(more )
Specimen
4A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
4B:
embryonic day 14.5
(more )
Specimen
4B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
4C:
embryonic day 14.5
(more )
Specimen
4C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: rib cartilage condensation |
Present |
Regionally restricted |
4C
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Expression restricted to perichondrium surrounding cartilaginous ribs and in neighboring mesenchymal cells. No expression in chondrocytes.
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Specimen
4E/F:
embryonic day 18.5
(more )
Specimen
4E/F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: vertebra |
Strong |
Regionally restricted |
4E/F
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High expression in fibroblasts of periosteum and osteoblasts of ossified vertebrae.
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Specimen
4G:
embryonic day 18.5
(more )
Specimen
4G:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Strong |
Regionally restricted |
4G
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Particularly strong expression in periosteum and in osteoblasts of mandibular arches.
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Specimen
4H/I:
embryonic day 18.5
(more )
Specimen
4H/I:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: incisor dental papilla |
Strong |
Regionally restricted |
4H/I
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High level of expression in developing teeth, particulary in odontoblasts. Expression also detected in pulp cells.
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Specimen
6A/B:
embryonic day 16.5
(more )
Specimen
6A/B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: connective tissue |
Present |
Regionally restricted |
6A/B
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Authors report expression in developing ligaments and tendons.
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Specimen
6C/D:
embryonic day 16.5
(more )
Specimen
6C/D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: umbilical cord |
Present |
Regionally restricted |
6C/D
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Authors report robust expression in vessel walls of the umbilical cord.
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Specimen
6E:
embryonic day 16.5
(more )
Specimen
6E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
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Specimen
6F:
embryonic day 14.5
(more )
Specimen
6F:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
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Specimen
6G/H:
embryonic day 18.5
(more )
Specimen
6G/H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
10% Formalin
|
Embedding:
|
Paraffin
|
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