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Gene Expression Data
Assay Details
Assay
Reference: J:124197 Kiyozumi D, et al., Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1. Matrix Biol. 2007 Jul;26(6):456-462
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5578614
Gene symbol: Frem1
Gene name: Fras1 related extracellular matrix protein 1
Antibody: Anti-QBRICK
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Cryosections were fixed in absolute ethanol.
Results
Specimen 2B: postnatal week 9 (more )
Note: Double labeled: green-Frem1; red-Lamc1 (assay MGI:5578519).
Structure Level Pattern Image Note
TS28: pigmented retinal epithelium Trace Regionally restricted 2B QBRICK/Frem1 Expression is at the basement membrane.
TS28: retina ganglion cell layer Absent 2B QBRICK/Frem1

Specimen 2C: embryonic day 14.5 (more )
Note: Double labeled: green-Frem1; red-Lamc1 (assay MGI:5578519).
Structure Level Pattern Image Note
TS22: neural retinal epithelium Present Regionally restricted 2C QBRICK/Frem1 Expression is at the basement membrane.
TS22: pigmented retinal epithelium Present Regionally restricted 2C QBRICK/Frem1 Expression is at the basement membrane.

Specimen 2D: postnatal adult (more )
Note: Double labeled: green-Frem1; red-Lamc1 (assay MGI:5578519).
Structure Level Pattern Image Note
TS28: salivary gland Trace 2D QBRICK/Frem1
TS28: salivary duct Absent 2D QBRICK/Frem1

Specimen 2E: embryonic day 14.5 (more )
Note: Double labeled: green-Frem1; red-Lamc1 (assay MGI:5578519).
Structure Level Pattern Image Note
TS22: metanephros Present Regionally restricted 2E QBRICK/Frem1 Expression is at the renal epithelial basement membrane.

Specimen 3A: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: epidermis Present Regionally restricted 3A QBRICK/Frem1 Expression is at the interfollicular epidermal basement membrane.
TS25: hair follicle Strong Regionally restricted 3A QBRICK/Frem1 Expression is at the basement membrane engulfing dermal papilla.

Specimen 3B: postnatal week 4 (more )
Note: Double labeled: green-Frem1; red-Lamc1 (assay MGI:5578519).
Structure Level Pattern Image Note
TS28: coat hair follicle Present Regionally restricted 3B QBRICK/Frem1 Expression is at the basement membrane engulfing dermal papilla.
TS28: epidermis Trace Regionally restricted 3B QBRICK/Frem1 Expression is at the interfollicular epidermal basement membrane.
TS28: hair outer root sheath Trace Regionally restricted 3B QBRICK/Frem1 Expression is at the basement membrane.

Specimen 4A Qbrick/Frem1+/-: postnatal newborn; Frem1tm1Ksek/Frem1+ (more )
Structure Level Pattern Image Note
TS27: epidermis Present Regionally restricted 4A Qbrick/Frem1+/- QBRICK/Frem1 Expression is at the basement membrane.

Specimen 4A Qbrick/Frem1-/-: postnatal newborn; Frem1tm1Ksek/Frem1tm1Ksek (more )
Structure Level Pattern Image Note
TS27: epidermis Weak Regionally restricted 4A Qbrick/Frem1-/- QBRICK/Frem1 Expression is at the basement membrane.

Specimen 4A Frem2my/+: postnatal newborn; Frem2my/Frem2+ (more )
Structure Level Pattern Image Note
TS27: epidermis Present Regionally restricted 4A Frem2my/+ QBRICK/Frem1 Expression is at the basement membrane.

Specimen 4A Frem2my/my: postnatal newborn; Frem2my/Frem2my (more )
Structure Level Pattern Image Note
TS27: epidermis Weak Regionally restricted 4A Frem2my/my QBRICK/Frem1 Expression is at the basement membrane.

Specimen 4A Grip1eb/+: postnatal newborn; Grip1eb/Grip1+ (more )
Structure Level Pattern Image Note
TS27: epidermis Present Regionally restricted 4A Grip1eb/+ QBRICK/Frem1 Expression is at the basement membrane.

Specimen 4A Grip1eb/eb: postnatal newborn; Grip1eb/Grip1eb (more )
Structure Level Pattern Image Note
TS27: epidermis Weak Regionally restricted 4A Grip1eb/eb QBRICK/Frem1 Expression is at the basement membrane.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/28/2022
MGI 6.20
The Jackson Laboratory