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Gene Expression Data
Assay Details
Assay
Reference: J:133947 Summerhurst K, et al., 3D representation of Wnt and Frizzled gene expression patterns in the mouse embryo at embryonic day 11.5 (Ts19). Gene Expr Patterns. 2008 May;8(5):331-48
Assay type: RNA in situ
MGI Accession ID: MGI:5448021
Gene symbol: Wnt5a
Gene name: wingless-type MMTV integration site family, member 5A
Probe: Wnt5a cDNA14
Probe preparation: Antisense RNA
Assay notes: These data are obtained from optical projection tomography (OPT).
Results
Specimen 1D: embryonic day 11.5 (more )
Note: Left side is OPT reconstructed data, right side is the corresponding whole-mount in situ hybridized embryo.
Structure Level Pattern Image Note
TS19: branchial arch Present 1D
TS19: forebrain Present 1D
TS19: forelimb bud Present 1D
TS19: midbrain ventricular layer Present Regionally restricted 1D Expression is ventral and in a restricted domain dorsally.
Specimen Table 1: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 1st branchial arch Present
TS19: 2nd branchial arch Present
TS19: 3rd branchial arch Present
TS19: 4th branchial arch Present
TS19: alimentary system Present
TS19: forelimb bud mesenchyme Present
TS19: fronto-nasal process Present
TS19: handplate apical ectodermal ridge Present
TS19: head mesenchyme Present
TS19: hindbrain Present
TS19: lung Present
TS19: midbrain Present
TS19: reproductive system Present
TS19: urinary system Present
Specimen 3E: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 3E
Specimen 3F: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: forelimb bud Present 3F
Specimen 4E: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: physiological umbilical hernia Present Regionally restricted 4E There is asymmetric expression in the mesenchyme to one side of the midgut in the umbilical hernia.
Specimen 4F: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: midbrain-hindbrain junction Present Regionally restricted 4F Fine ventricular staining.
TS19: hindbrain ventricular layer Strong Regionally restricted 4F Anterior hindbrain.
Specimen 4G: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: hindbrain ventricular layer Present Regionally restricted 4G At the level of the otic vesicle within the hindbrain there is a very broad ventricular domain, excluding the floor plate, with territories of different dorsoventral levels.
TS19: hindbrain floor plate Absent 4G
Specimen 4H: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: hindbrain Present Regionally restricted 4H In the most posterior part of the hindbrain expression is restricted to two patches just dorsal to the floor plate.
Specimen 5 first: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a first Expression is in the proximal bud.
Specimen 5 second: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a second
Specimen 5 third: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a third
Specimen 5 fourth: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a fourth
Specimen 5 fifth: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a fifth
Specimen 5 sixth: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a sixth
Specimen 5 seventh: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 5_Wnt5a seventh
Specimen Table 2: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Including the progress zone.
TS19: handplate apical ectodermal ridge Present
Specimen Table S1: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted Broad ventricular expression is in ventral half of the mesencephalon. More restricted in ventricular zone dorsally.
TS19: midbrain-hindbrain junction Present Regionally restricted Fine ventricular expression through midbrain/hindbrain boundary.
TS19: hindbrain ventricular layer Present Regionally restricted Anterior to the otic vesicle.
TS19: hindbrain Present Regionally restricted Expression is at different levels along the dorsoventral axis, floor plate excluded. In the most posterior part of the hindbrain it is restricted to two ventral patches just dorsal to the floor plate.
TS19: head mesenchyme Present Regionally restricted Mesenchyme dorsolateral to otic vesicle.
TS19: eye Present Regionally restricted Optic cup.
TS19: physiological umbilical hernia Present Regionally restricted Expression is in mesenchyme, polarized to one side of midgut in umbilical hernia
TS19: stomach mesenchyme Present Regionally restricted Anterior.
TS19: branchial arch mesenchyme Present
TS19: lung mesenchyme Present
TS19: hindbrain floor plate Absent
Specimen Not shown E11.5: embryonic day 11.5 (more )
Note: virtual section
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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