Antibody:	CT-Neog
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		1C , 1A
TS22: hippocampus	Present	Regionally restricted	1A , 1C	Expression was detected on the surface of dividing cells at the basal boundary of the ventricular zone, co-expressed in cells with PCNA-positive nuclei.
TS22: telencephalon septum	Present	Regionally restricted	1A , 1C	Expression was detected on the surface of dividing cells at the basal boundary of the ventricular zone, co-expressed in cells with PCNA-positive nuclei.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	1F , 1D	Expression was detected in the neurogenic progenitor cells in the ventricular zone. The cells were also immunopositive for Nestin.

Structure	Level	Pattern	Image	Note
TS22: olfactory cortex ventricular layer	Present	Regionally restricted	1I , 1G	Expression was detected on the surface of dividing cells at the basal boundary of the ventricular zone, co-expressed in cells with PCNA-positive nuclei.
TS22: telencephalon septum	Present	Regionally restricted	1I , 1G	Expression was detected on the surface of dividing cells at the basal boundary of the ventricular zone, co-expressed in cells with PCNA-positive nuclei.

Structure	Level	Pattern	Image	Note
TS22: olfactory cortex ventricular layer	Present	Regionally restricted	1L , 1J	Expression was detected in the neurogenic progenitors within the ventricular zone. The cells were also immunopositive for Nestin.
TS22: thalamus ventricular layer	Present	Regionally restricted	1L , 1J	Expression was detected in the neurogenic progenitors within the ventricular zone. The cells were also immunopositive for Nestin.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted		Expression was detected in the radial glial cells in the ventricular zone. The cells were also immunopositive for GLAST.
TS22: olfactory cortex ventricular layer	Present	Regionally restricted		Expression was detected in the radial glial cells in the ventricular zone. The cells were also immunopositive for GLAST.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	2B , 2A	Expression was detected along the radial processes of dividing cells.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted	2D , 2C	Expression was detected along the radial processes of PCNA-positive dividing cells in the dorsal midbrain.
TS22: midbrain mantle layer	Absent		2C , 2D	Expression was absent from maturing neurons residing the mantle regions.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted	2E , 2F	Expression was detected in Nestin-positive cells in the ventricular zone of the dorsal midbrain.
TS22: midbrain	Present	Regionally restricted	2F , 2E	Expression was detected in the radial glial processes spanning the more immature dorso-caudal midbrain.

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Present	Regionally restricted		Expression was detected in GLAST-positive cells in the ventricular zone of the dorsal midbrain.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present		2H , 2G	Expression was detected in Nestin-positive processes of the neurogenic radial glial cells spanning the medulla oblongata.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present			Expression was detected in Nestin-positive cells in the cerebellum.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Present	Regionally restricted	2I	Expression was detected in cell bodies and radial processes of dividing gliogenic progenitors in the ventral ventricular zone of the spinal cord.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Present	Regionally restricted	3A , 3C	Expression was restricted to proliferative zones with PCNA-positive dividing cells.
TS25: corpus striatum	Present	Regionally restricted	3A , 3C	Expression was restricted to proliferative zones with PCNA-positive dividing cells.
TS25: hippocampus	Present	Regionally restricted	3A , 3C	Expression was restricted to proliferative zones with PCNA-positive dividing cells.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Present	Regionally restricted	3D , 3F	Expression was detected in Nestin-positive gliogenic precursors.
TS25: corpus striatum	Present	Regionally restricted	3D , 3F	Expression was detected in Nestin-positive gliogenic precursors.
TS25: hippocampus	Present	Regionally restricted	3D , 3F	Expression was detected in Nestin-positive gliogenic precursors.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Present	Regionally restricted	3I , 3G	Expression was detected in GLAST-positive gliogenic precursors.
TS25: corpus striatum	Present	Regionally restricted	3I , 3G	Expression was detected in GLAST-positive gliogenic precursors.
TS25: hippocampus	Present	Regionally restricted	3I , 3G	Expression was detected in GLAST-positive gliogenic precursors.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle subependymal layer	Present		4A

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle subependymal layer	Present	Regionally restricted	4C , 4E	Expression was detected on the cell bodies and processes in the subventricular zone of the anterior corner of the lateral ventricle, co-labeling with Nestin. Expression was in neural stem cells (Type B) in the adult subventricular zone.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle subependymal layer	Present	Regionally restricted	4H , 4F	Expression was detected on the cell bodies and processes in the subventricular zone of the anterior corner of the lateral ventricle, co-labeling with GFAP. Expression was in neural stem cells (Type B) in the adult subventricular zone.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present		S1A

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present		S1C

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present		S1E
TS22: midbrain	Present		S1E

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	S2A , S2C	Expression was absent from BetaIII-tubulin-positive maturing neurons in the mantle regions of the dorsal and ventral forebrain.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	S2D , S2F	Expression was absent from BetaIII-tubulin-positive maturing neurons in the mantle regions of the dorsal and ventral forebrain.