Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:90402
Merrill BJ, et al., Tcf3: a transcriptional regulator of axis induction in the early embryo. Development. 2004 Jan;131(2):263-74
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:4943175
|
|
Gene symbol:
|
Cer1
|
|
Gene name:
|
cerberus 1, DAN family BMP antagonist
|
|
Probe:
|
Cer1 probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
6A:
embryonic day 7.0
(more  )
Note: Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
Specimen
6A:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Data for +/+ or +/- embryos were indistinguishable and are referred to as 'wild-type'; thus the pictured specimen could have been a heterozygous animal.
|
Specimen
Heterozygote:
embryonic day 7.0; Tcf7l1tm2Efu/Tcf7l1 +
(more )
Specimen
Heterozygote:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Tcf7l1tm2Efu/Tcf7l1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6B:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
|
Mutant Allele(s):
|
Tcf7l1tm2Efu/Tcf7l1tm2Efu
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: anterior visceral endoderm |
Present |
Regionally restricted |
6B
|
In mutants, as in wild type, expression is present in the anterior visceral endoderm. However, in mutants, unlike in wild type, expression is also expanded to the posterior endoderm.
|
| TS10: posterior visceral endoderm |
Present |
Regionally restricted |
6B
|
In mutants, as in wild type, expression is present in the anterior visceral endoderm. However, in mutants, unlike in wild type, expression is also expanded to the posterior endoderm.
|
Specimen
6B':
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6
|
|
Mutant Allele(s):
|
Tcf7l1tm2Efu/Tcf7l1tm2Efu
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: anterior visceral endoderm |
Present |
Regionally restricted |
6B'
|
In mutants, as in wild type, expression is present in the anterior visceral endoderm. However, in mutants, unlike in wild type, expression is also expanded to the posterior endoderm.
|
| TS10: posterior visceral endoderm |
Present |
Regionally restricted |
6B'
|
In mutants, as in wild type, expression is present in the anterior visceral endoderm. However, in mutants, unlike in wild type, expression is also expanded to the posterior endoderm.
|
|
Analysis Tools