Probe:	UI-M-BH0-aji-a-11-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_u	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_v	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_w	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_x	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Wnt7b-Shh E11.5_sagittal , Wnt7b-Shh E11.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Wnt7b-Shh E11.5_sagittal , Wnt7b-Shh E11.5_coronal	Staining is strong in the VZ of the prethalamus, PVH nucleus, AVH, posterior ID, premam zone & TT, moderate in ventral POA, weak in dorsal thalamus & dorsal POA & either absent or ubiquitous in the anterior ID, VMH & ARH nuclei & mam & supramam zones.
TS19: telencephalon mantle layer	Weak	Non-Uniform	Wnt7b-Shh E11.5_sagittal , Wnt7b-Shh E11.5_coronal	There is weak staining in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Weak	Non-Uniform	Wnt7b-Shh E11.5_coronal , Wnt7b-Shh E11.5_sagittal	There is weak staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Moderate		Wnt7b-Shh E12.5_coronal , Wnt7b-Shh E12.5_sagittal	There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Wnt7b-Shh E12.5_sagittal , Wnt7b-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Moderate		Wnt7b-Shh E12.5_coronal , Wnt7b-Shh E12.5_sagittal	There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Wnt7b-Shh E12.5_coronal , Wnt7b-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Strong	Non-Uniform	Wnt7b-Shh E12.5_sagittal , Wnt7b-Shh E12.5_coronal	There is strong staining in the ventricular zone of the premam zone and the TT and moderate staining in the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS20: ganglionic eminence	Weak	Non-Uniform	Wnt7b-Shh E12.5_sagittal , Wnt7b-Shh E12.5_coronal	There is weak staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		Wnt7b-Shh E12.5_coronal , Wnt7b-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Very strong	Non-Uniform	Wnt7b-Shh E12.5_sagittal , Wnt7b-Shh E12.5_coronal	There is very strong staining in the VZ of the dorsal POA and PVH nucleus and moderate staining in the ventral POA and the AVH. Staining is either absent or ubiquitous in the VZ of the anterior and posterior IDs and VMH and ARH nuclei.
TS20: thalamus mantle layer	Ambiguous		Wnt7b-Shh E12.5_coronal , Wnt7b-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Very strong	Non-Uniform	Wnt7b-Shh E12.5_coronal , Wnt7b-Shh E12.5_sagittal	There is very strong staining in the ventricular zone of the prethalamus and moderate staining in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Strong		Wnt7b-Shh E14.5_sagittal , Wnt7b-Shh E14.5_coronal	There is strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Strong		Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	There is strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Strong	Non-Uniform	Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	There is strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Strong	Non-Uniform	Wnt7b-Shh E14.5_sagittal , Wnt7b-Shh E14.5_coronal	There is strong staining in the ventricular zone of the premam zone and the TT. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS22: ganglionic eminence	Weak	Non-Uniform	Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	There is weak staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Very strong	Non-Uniform	Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	There is very strong staining in the VZ of the PVH nucleus & the AVH, moderate staining in the dorsal POA & weak staining in the ventral POA. Staining is either absent or ubiquitous in the VZ of the anterior & posterior IDs & the VMH & ARH nuclei.
TS22: thalamus mantle layer	Ambiguous		Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Very strong	Non-Uniform	Wnt7b-Shh E14.5_coronal , Wnt7b-Shh E14.5_sagittal	There is very strong staining in the ventricular zone of the prethalamus and moderate staining in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Wnt7b-Shh E16.5_sagittal , Wnt7b-Shh E16.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Very strong		Wnt7b-Shh E16.5_sagittal , Wnt7b-Shh E16.5_coronal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Wnt7b-Shh E16.5_coronal , Wnt7b-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Wnt7b-Shh E16.5_sagittal , Wnt7b-Shh E16.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Strong	Non-Uniform	Wnt7b-Shh E16.5_sagittal , Wnt7b-Shh E16.5_coronal	There is strong staining in the ventricular zone of the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS24: ganglionic eminence	Weak	Non-Uniform	Wnt7b-Shh E16.5_coronal , Wnt7b-Shh E16.5_sagittal	There is weak staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Wnt7b-Shh E16.5_sagittal , Wnt7b-Shh E16.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Strong	Non-Uniform	Wnt7b-Shh E16.5_sagittal , Wnt7b-Shh E16.5_coronal	There is strong staining in the VZ of the PH and moderate staining in the dorsal and ventral POAs, the PVH and SCH nuclei, the AVH, the DAH and the PH. Staining is either absent or ubiquitous in the VZ of the DMH, VMH and ARH nuclei.
TS24: thalamus mantle layer	Ambiguous		Wnt7b-Shh E16.5_coronal , Wnt7b-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Very strong	Non-Uniform	Wnt7b-Shh E16.5_coronal , Wnt7b-Shh E16.5_sagittal	There is very strong staining in the ventricular zone of the prethalamus and moderate staining in the dorsal thalamus.