Probe:	UI-M-AL0-abx-a-08-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_k	Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_l	Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_m	Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_n	Expression is present in a dorsal domain that encompasses the eminentia thalami, prethalamus and the paraventricular hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Dkk3-Shh-E11.5-sagittal , Dkk3-Shh E11.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Dkk3-Shh E11.5-coronal , Dkk3-Shh-E11.5-sagittal	There is strong staining in the VZ of the prethalamus, PVH nucleus & the AVH. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, dorsal & ventral POAs, VMH & ARH nuclei, anterior & posterior IDs, premam, mam & supramam zones & TT.
TS19: telencephalon mantle layer	Ambiguous		Dkk3-Shh E11.5-coronal , Dkk3-Shh-E11.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Strong	Non-Uniform	Dkk3-Shh-E11.5-sagittal , Dkk3-Shh E11.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex and weak staining in the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Strong		Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Ambiguous		Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Very strong	Non-Uniform	Dkk3-SHH-E12.5-sagittal , Dkk3-Shh E12.5-coronal	There is very strong staining in the ventricular zone of the PVH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Ambiguous		Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Very strong	Non-Uniform	Dkk3-Shh E12.5-coronal , Dkk3-SHH-E12.5-sagittal	There is very strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Strong		Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Very strong	Non-Uniform	Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal	There is very strong staining in the ventricular zone of the premam zone and the TT. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS22: ganglionic eminence	Ambiguous		Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH , VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Very strong	Non-Uniform	Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal	There is very strong staining in the ventricular zone of the dorsal and ventral POAs, the PVH and VMH nuclei, the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the ARH nucleus.
TS22: thalamus mantle layer	Ambiguous		Dkk3-Shh E14.5-coronal , Dkk3-SHH-E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Very strong		Dkk3-SHH-E14.5-sagittal , Dkk3-Shh E14.5-coronal	There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Very strong		Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	There is very strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Very strong		Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Very strong		Dkk3-Shh E16.5-coronal , Dkk3-SHH-E16.5-sagittal	There is very strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Very strong	Non-Uniform	Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS24: ganglionic eminence	Moderate	Non-Uniform	Dkk3-Shh E16.5-coronal , Dkk3-SHH-E16.5-sagittal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Dkk3-Shh E16.5-coronal , Dkk3-SHH-E16.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Very strong		Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Very strong		Dkk3-SHH-E16.5-sagittal , Dkk3-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.