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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450785
Gene symbol: Magel2
Gene name: MAGE family member L2
Probe: MGC clone 78154
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Magel2 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Strong Non-Uniform Magel2-Shh E11.5_sagittal , Magel2-Shh E11.5_coronal , Magel2-Shh E11.5_sagittal-2 Staining is strong in the MZ of the dorsal & ventral POAs, PVH, VMH & ARH nuclei, AVH, anterior & posterior IDs, premam, mam & supramam zones & TT & moderate in the prethalamus. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus.
TS19: diencephalon lateral wall ventricular layer Ambiguous Magel2-Shh E11.5_sagittal , Magel2-Shh E11.5_coronal , Magel2-Shh E11.5_sagittal-2 Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Strong Non-Uniform Magel2-Shh E11.5_sagittal-2 , Magel2-Shh E11.5_sagittal , Magel2-Shh E11.5_coronal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer Ambiguous Magel2-Shh E11.5_sagittal , Magel2-Shh E11.5_coronal , Magel2-Shh E11.5_sagittal-2 Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Magel2 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Magel2-Shh E12.5_coronal , Magel2-Shh E12.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Magel2-Shh E12.5_coronal , Magel2-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Strong Non-Uniform Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal There is strong staining in the mantle zone of the premam and mam zones and the TT and weak staining in the supramam zone.
TS20: diencephalon ventricular layer Ambiguous Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Strong Non-Uniform Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer Strong Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal There is strong staining in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous Magel2-Shh E12.5_coronal , Magel2-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Moderate Non-Uniform Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal There is moderate staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS20: thalamus ventricular layer Ambiguous Magel2-Shh E12.5_sagittal , Magel2-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Magel2 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous Magel2-Shh E14.5_coronal , Magel2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous Magel2-Shh E14.5_coronal , Magel2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous Magel2-Shh E14.5_sagittal , Magel2-Shh E14.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Strong Non-Uniform Magel2-Shh E14.5_sagittal , Magel2-Shh E14.5_coronal There is strong staining in the mantle zone of the premam and mam zones and the TT and weak staining in the supramam zone.
TS22: diencephalon ventricular layer Ambiguous Magel2-Shh E14.5_sagittal , Magel2-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Strong Non-Uniform Magel2-Shh E14.5_sagittal , Magel2-Shh E14.5_coronal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Strong Magel2-Shh E14.5_coronal , Magel2-Shh E14.5_sagittal There is strong staining in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous Magel2-Shh E14.5_coronal , Magel2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Moderate Non-Uniform Magel2-Shh E14.5_coronal , Magel2-Shh E14.5_sagittal There is moderate staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS22: thalamus ventricular layer Ambiguous Magel2-Shh E14.5_sagittal , Magel2-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Magel2 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous Magel2-Shh E16.5_coronal , Magel2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous Magel2-Shh E16.5_coronal , Magel2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous Magel2-Shh E16.5_coronal , Magel2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Strong Non-Uniform Magel2-Shh E16.5_coronal , Magel2-Shh E16.5_sagittal There is strong staining in the mantle zone of the premam and mam zones and moderate staining in the supramam zone.
TS24: diencephalon ventricular layer Ambiguous Magel2-Shh E16.5_coronal , Magel2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Moderate Non-Uniform Magel2-Shh E16.5_sagittal , Magel2-Shh E16.5_coronal There is moderate staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer Strong Magel2-Shh E16.5_sagittal , Magel2-Shh E16.5_coronal There is strong staining in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous Magel2-Shh E16.5_sagittal , Magel2-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Moderate Non-Uniform Magel2-Shh E16.5_sagittal , Magel2-Shh E16.5_coronal There is moderate staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS24: thalamus ventricular layer Ambiguous Magel2-Shh E16.5_coronal , Magel2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.


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last database update
06/28/2022
MGI 6.20
The Jackson Laboratory