Assay
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Reference:
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J:157819
Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4450731
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Gene symbol:
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Sema5a
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Gene name:
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sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
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Probe:
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UI-M-BH2.3-aoj-b-09-0-UI.s1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
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Results
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Specimen
Sema5a - E11.5:
embryonic day 11.5
(more )
Specimen
Sema5a - E11.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon lateral wall mantle layer |
Moderate |
Non-Uniform |
Sema5a-Shh E11.5_coronal
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Staining is moderate in the MZ of the dorsal thalamus & weak in the AVH. Staining is either absent or ubiquitous in the MZ of the prethalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, anterior & posterior IDs, premam, mam & supramam zones & TT.
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TS19: diencephalon lateral wall ventricular layer |
Ambiguous |
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Sema5a-Shh E11.5_coronal
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Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
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TS19: telencephalon mantle layer |
Ambiguous |
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Sema5a-Shh E11.5_coronal
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Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
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TS19: telencephalon ventricular layer |
Very strong |
Non-Uniform |
Sema5a-Shh E11.5_coronal
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There is very strong staining in the ventricular zone of the cerebral cortex and moderate staining in the ganglionic eminences.
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Specimen
Sema5a - E12.5:
embryonic day 12.5
(more )
Specimen
Sema5a - E12.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Sema5a - E14.5:
embryonic day 14.5
(more )
Specimen
Sema5a - E14.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Sema5a - E16.5:
embryonic day 16.5
(more )
Specimen
Sema5a - E16.5:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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