Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143598
Jaks V, et al., Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat Genet. 2008 Nov;40(11):1291-9
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3832645
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Gene symbol:
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Lgr5
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Gene name:
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leucine rich repeat containing G protein coupled receptor 5
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Specimens were fixed in 2% paraformaldehyde and 0.2% glutaraldehyde.
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Results
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Specimen
S1a:
(close  )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
S1a
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Expression in the outer root sheath of larger forming hair follicles.
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| TS26: hair root sheath |
Present |
Regionally restricted |
S1a
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Expression in the outer root sheath of larger forming hair follicles.
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Specimen
S1b:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hair follicle |
Present |
Regionally restricted |
S1b
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Expression in lower part of outer root sheath of hair follicles in dorsal skin.
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| TS26: hair root sheath |
Present |
Regionally restricted |
S1b
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Expression in lower part of outer root sheath of hair follicles in dorsal skin.
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Specimen
S1c:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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|
Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: vibrissa |
Present |
Regionally restricted |
S1c
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Expression in the outer cell layer of the vibrissa bulb.
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Specimen
1a:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Anagen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair outer root sheath |
Present |
Regionally restricted |
1a
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Expression localized to the lower part of the outer root sheath surrounding the hair follicle.
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Specimen
1b:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Catagen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1b
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Expressing cells link the mesenchymal component of the hair follicle (the dermal papilla) with the bulge area.
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Specimen
1c:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal
|
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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|
Embedding:
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Paraffin
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|
Note:
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Telogen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1c
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Expression in the lower bulge and secondary germ.
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Specimen
1g:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal
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Sex:
|
Not Specified
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Type:
|
section
|
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1g
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Expression concentrated in the lower bulge, and in the area between the dermal papilla and bulge (the secondary germ.
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| TS28: dermis papillary layer |
Absent |
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1g
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Authors state expression was in cells distinct from the vimentin-positive dermal papilla cells.
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Specimen
1h:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
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Age:
|
postnatal week 8
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
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Note:
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Telogen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1h
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Expression mostly in newly formed hair follicle, but not in the club hair.
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Specimen
1j:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Anagen phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1j
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Authors state expression does not overlap with that of CD34.
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Specimen
1k:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
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postnatal
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|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1k
|
Authors report partial overlap of expression with that of CD34.
|
Specimen
1m:
(close )
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Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
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|
Type:
|
section
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|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1m
|
Authors state expression does not overlap with that of K15.
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Specimen
1n:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1n
|
Authors report partial overlap of expression with that of K15 in the bulge.
|
Specimen
1p:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1p
|
Authors state that expression is in cells distinct from that staining by the MTS24 antibody, which recognizes the Plet-1 glycoprotein.
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Specimen
1q:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal
|
|
Sex:
|
Not Specified
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|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
1q
|
Authors state expression is in cells distinct from those staining by the MTS24 antibody (recognizing the Plet-1 glycoprotein).
|
Specimen
2a:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
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Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
Specimen
2b:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Pre-anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2b
|
Some expression in the secondary germ area.
|
Specimen
2c:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
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Early anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2c
|
Expression in the secondary germ area.
|
Specimen
2d:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
Specimen
2e:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair bulb |
Present |
Regionally restricted |
2e
|
Expression in the bulb in the outer cell layer.
|
Specimen
2f:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair bulb |
Present |
Regionally restricted |
2f
|
Expression in the bulb in the outer cell layer.
|
Specimen
2g:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair bulb |
Present |
Regionally restricted |
2g
|
Expression in the bulb in the outer cell layer.
|
Specimen
2h:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Late anagen phase.
|
Specimen
2i:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Late anagen phase.
|
Specimen
2j:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
Specimen
2k:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
Specimen
2l:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Anagen phase.
|
Specimen
2n:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2n
|
Expression in the bulge area of club hair.
|
Specimen
2o:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2o
|
Expression in the bulge area of club hair.
|
Specimen
2p:
(close )
|
Genetic Background:
|
involves: 129S5/SvEvBrd
|
|
Mutant Allele(s):
|
Lgr5tm1Ah/Lgr5tm1Ah
|
|
Age:
|
postnatal week 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Telogen phase.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hair follicle |
Present |
Regionally restricted |
2p
|
Expression in the bulge area of club hair.
|
|
Analysis Tools
)