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Gene Expression Data
Assay Details
Assay
Reference: J:142671 Kalsotra A, et al., A postnatal switch of CELF and MBNL proteins reprograms alternative splicing in the developing heart. Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20333-8
Assay type: RT-PCR
MGI Accession ID: MGI:3831849
Gene symbol: Popdc2
Gene name: popeye domain containing 2
Probe: Popdc2-pC, Popdc2-pD
Assay notes: Total RNA was isolated from 6-20 pooled hearts at the indicated time points. The data were plotted both as percentage splicing inclusion of the variable region(s) to show the absolute change and as the percentage of total change to identify the timing ofmaximal change.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
E14 E14.0 (a) TS22: heart Present 0.5 µg; total RNA FVB Not Specified
E18 E18.0 (a) TS26: heart Present 0.5 µg; total RNA FVB Not Specified
PN1 P1 TS27: heart Present 0.5 µg; total RNA FVB Not Specified
PN3 P3 TS27: heart Present 0.5 µg; total RNA FVB Not Specified
Adult P m 3 TS28: heart Present 0.5 µg; total RNA FVB Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There was a biphasic increase in splicing transitions in which more than 20% of the total change took place between E14 and E18, less than 20% occured between E18 and PN1, and more than 20% occured between PN1 and adult.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory