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Gene Expression Data
Assay Details
Assay
Reference: J:139062 Brooks WS, et al., G2E3 is a dual function ubiquitin ligase required for early embryonic development. J Biol Chem. 2008 Aug 8;283(32):22304-15
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3829448
Gene symbol: G2e3
Gene name: G2/M-phase specific E3 ubiquitin ligase
Reporter: lacZ
Direct Detection
Assay notes: Embryos were fixed in LacZ fixative (1% formaldehyde, 0.2% glutaraldehyde, 2mM MgCl2, 5 mM EGTA, 0.02% Nonidet P-40 in PBS).
Results
Specimen 7 E10.5 +/-: embryonic day 10.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS17: branchial arch Present 7_E10.5 +/-
TS17: forebrain Present 7_E10.5 +/-
TS17: future spinal cord Present 7_E10.5 +/-
TS17: limb Present 7_E10.5 +/-
TS17: trunk Present Diffuse 7_E10.5 +/- Expression is in the abdomen.

Specimen 7 E12.5 +/- top right: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: forebrain Very strong 7_E12.5 +/- top right
TS20: forelimb Very strong 7_E12.5 +/- top right
TS20: hindlimb Very strong 7_E12.5 +/- top right
TS20: spinal cord Very strong 7_E12.5 +/- top right
TS20: head Very strong Regionally restricted 7_E12.5 +/- top right Expression is in the branchial arch.
TS20: trunk Present Diffuse 7_E12.5 +/- top right Expression is in the abdomen.

Specimen 7 E12.5 +/- bottom left: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: forebrain Very strong 7_E12.5 +/- bottom left
TS20: head Very strong Regionally restricted 7_E12.5 +/- bottom left Expression is in the branchial arch.

Specimen 7 E12.5 +/- bottom right: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: hindlimb Very strong 7_E12.5 +/- bottom right
TS20: spinal cord Very strong 7_E12.5 +/- bottom right

Specimen 7 E14.5 +/- top right: embryonic day 14.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS22: forebrain Very strong 7_E14.5 +/- top right
TS22: midbrain Present 7_E14.5 +/- top right
TS22: spinal cord Present 7_E14.5 +/- top right
TS22: trunk Present Regionally restricted 7_E14.5 +/- top right Expression is in the abdomen.
TS22: limb Absent 7_E14.5 +/- top right

Specimen 7 E14.5 +/- bottom right: embryonic day 14.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS22: forebrain Very strong 7_E14.5 +/- bottom left

Specimen 8A: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: forebrain Present 8A

Specimen 8C: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 8C Expression is within the ependymal cells lining the ventricles and the underlying nervous tissue.

Specimen 8D: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Note: (D) is a higher power view of (C).
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 8D Expression is within the ependymal cells lining the ventricles and the underlying nervous tissue.

Specimen 8E: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: brain Present Regionally restricted 8E Expression is within the ependymal cells lining the ventricles and the underlying nervous tissue.

Specimen 8F: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: choroid plexus Present 8F

Specimen 8G: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: neural retinal epithelium Weak Diffuse 8G

Specimen 8H: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: intestine epithelium Present 8H

Specimen 8I: embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS20: intestine epithelium Present 8I

Specimen 8J: postnatal week 4; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS28: interstitium of the testis Present 8J
TS28: seminiferous tubule Weak 8J

Specimen 8K: postnatal week 4; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS28: ductus deferens Very strong Regionally restricted 8K Expression is in the apical cells.

Specimen 8L: postnatal adult; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Strong 8L
TS28: cerebellum granule cell layer Absent 8L
TS28: cerebellum molecular layer Absent 8L

Specimen Not shown: postnatal week 4; G2e3Gt(RRM192)Byg/G2e3+ (more )
Structure Level Pattern Image Note
TS28: heart Absent
TS28: liver Absent
TS28: metanephros Absent
TS28: pancreas Absent
TS28: spleen Absent


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory