Gene Expression Data
Assay Details
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Assay
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Reference:
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J:139062
Brooks WS, et al., G2E3 is a dual function ubiquitin ligase required for early embryonic development. J Biol Chem. 2008 Aug 8;283(32):22304-15
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3829448
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Gene symbol:
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G2e3
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Gene name:
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G2/M-phase specific E3 ubiquitin ligase
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Embryos were fixed in LacZ fixative (1% formaldehyde, 0.2% glutaraldehyde, 2mM MgCl2, 5 mM EGTA, 0.02% Nonidet P-40 in PBS).
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Results
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Specimen
7 E10.5 +/-:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 E12.5 +/- top right:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 E12.5 +/- bottom left:
embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3 +
(more  )
Specimen
7 E12.5 +/- bottom left:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 E12.5 +/- bottom right:
embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3 +
(more )
Specimen
7 E12.5 +/- bottom right:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 E14.5 +/- top right:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
7 E14.5 +/- bottom right:
embryonic day 14.5; G2e3Gt(RRM192)Byg/G2e3 +
(more )
Specimen
7 E14.5 +/- bottom right:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
8A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
8C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
8C
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Expression is within the ependymal cells lining the ventricles and the underlying nervous tissue.
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Specimen
8D:
embryonic day 12.5; G2e3Gt(RRM192)Byg/G2e3 +
(more )
Note: (D) is a higher power view of (C).
Specimen
8D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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(D) is a higher power view of (C).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
8D
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Expression is within the ependymal cells lining the ventricles and the underlying nervous tissue.
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Specimen
8E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
Regionally restricted |
8E
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Expression is within the ependymal cells lining the ventricles and the underlying nervous tissue.
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Specimen
8F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
8H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
8I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
8J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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postnatal week 4
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
8K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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postnatal week 4
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: ductus deferens |
Very strong |
Regionally restricted |
8K
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Expression is in the apical cells.
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Specimen
8L:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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G2e3Gt(RRM192)Byg/G2e3+
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Age:
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postnatal week 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Analysis Tools