Probe:	MH965 probe 844
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: alimentary system epithelium	Present		2	Expression is widespread.
TS22: central nervous system	Present		2	Expression is widespread.
TS22: metanephros	Present		2	Expression is widespread.
TS22: pancreas	Present		2	Expression is widespread.
TS22: peripheral nervous system	Present		2	Expression is widespread.
TS22: respiratory system epithelium	Present		2	Expression is widespread.
TS22: thymus primordium	Present		2	Expression is widespread.
TS22: vibrissa follicle	Present		2	Expression is widespread.
TS22: cranial ganglion	Present		2	There is a relative regional elevation of expression.
TS22: dorsal root ganglion	Present		2	There is a relative regional elevation of expression.
TS22: heart	Present		2	There is a relative regional elevation of expression.
TS22: intestine	Present		2	There is a relative regional elevation of expression.
TS22: liver	Present		2	There is a relative regional elevation of expression.
TS22: lung	Present		2	There is a relative regional elevation of expression.
TS22: neocortex	Present		2	There is a relative regional elevation of expression.
TS22: salivary gland	Present		2	There is a relative regional elevation of expression.
TS22: skeletal muscle	Present		2	There is a relative regional elevation of expression.
TS22: spinal cord	Present		2	There is a relative regional elevation of expression.
TS22: appendicular skeleton	Absent		2	There is no expression in the cartilage.
TS22: axial skeleton	Absent		2	There is no expression in the cartilage.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3B Idh3a
TS22: midbrain	Moderate		3B Idh3a
TS22: cerebellum	Moderate		3B Idh3a
TS22: pons	Strong		3B Idh3a
TS22: medulla oblongata	Strong		3B Idh3a
TS22: spinal cord	Strong		3B Idh3a
TS22: cranial ganglion	Strong		3B Idh3a
TS22: dorsal root ganglion	Strong		3B Idh3a
TS22: olfactory epithelium	Strong		3B Idh3a
TS22: intestine	Strong		3B Idh3a
TS22: salivary gland	Strong		3B Idh3a
TS22: tongue	Moderate		3B Idh3a
TS22: stomach	Moderate		3B Idh3a
TS22: liver	Strong		3B Idh3a
TS22: lung	Strong		3B Idh3a
TS22: metanephros	Strong		3B Idh3a
TS22: thyroid gland	Strong		3B Idh3a
TS22: pituitary gland	Moderate		3B Idh3a
TS22: pancreas	Strong		3B Idh3a
TS22: adrenal gland	Moderate		3B Idh3a
TS22: heart	Strong		3B Idh3a
TS22: skeletal muscle	Moderate		3B Idh3a
TS22: cartilage	Absent		3B Idh3a	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Idh3a	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Strong		MH965_4C , MH965_6A	There is broad expression, with transcripts being observed in nearly all tissues.
TS22: heart	Strong		MH965_6A , MH965_4C
TS22: intestine epithelium	Strong		MH965_6A , MH965_4C
TS22: liver	Strong		MH965_6A , MH965_4C
TS22: peripheral nervous system	Strong		MH965_6A , MH965_4C
TS22: skeletal muscle	Strong		MH965_6A , MH965_4C
TS22: thymus primordium	Strong		MH965_6A , MH965_4C
TS22: vibrissa follicle	Strong		MH965_6A , MH965_4C
TS22: skeleton	Absent		MH965_6A , MH965_4C	There is no expression in the developing axial and appendicular cartilage.