Probe:	MH1441 probe 1149
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3B Aco1
TS22: midbrain	Weak		3B Aco1
TS22: cerebellum	Weak		3B Aco1
TS22: pons	Strong		3B Aco1
TS22: medulla oblongata	Moderate		3B Aco1
TS22: spinal cord	Moderate		3B Aco1
TS22: cranial ganglion	Strong		3B Aco1
TS22: dorsal root ganglion	Strong		3B Aco1
TS22: olfactory epithelium	Weak		3B Aco1
TS22: intestine	Weak		3B Aco1
TS22: salivary gland	Strong		3B Aco1
TS22: tongue	Weak		3B Aco1
TS22: stomach	Moderate		3B Aco1
TS22: liver	Strong		3B Aco1
TS22: lung	Strong		3B Aco1
TS22: metanephros	Strong		3B Aco1
TS22: thyroid gland	Weak		3B Aco1
TS22: pituitary gland	Weak		3B Aco1
TS22: pancreas	Moderate		3B Aco1
TS22: adrenal gland	Weak		3B Aco1
TS22: heart	Weak		3B Aco1
TS22: skeletal muscle	Weak		3B Aco1
TS22: cartilage	Absent		3B Aco1	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Aco1	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		MH1441_3B , MH1441_5B	This gene is broadly expressed.
TS22: adrenal gland	Present		MH1441_3B , MH1441_5B	This is one of the tissues with elevated expression levels.
TS22: liver	Present		MH1441_3B , MH1441_5B	This is one of the tissues with elevated expression levels.
TS22: lung	Present		MH1441_3B , MH1441_5B	This is one of the tissues with elevated expression levels.
TS22: metanephros	Present		MH1441_3B , MH1441_5B	This is one of the tissues with elevated expression levels.
TS22: salivary gland	Present		MH1441_3B , MH1441_5B	This is one of the tissues with elevated expression levels.
TS22: brainstem	Present		MH1441_5B , MH1441_3B	This is one of the nervoys system tissues with elevated expression levels.
TS22: cranial ganglion	Present		MH1441_5B , MH1441_3B	This is one of the nervoys system tissues with elevated expression levels.
TS22: dorsal root ganglion	Present		MH1441_5B , MH1441_3B	This is one of the nervoys system tissues with elevated expression levels.
TS22: forebrain	Present		MH1441_5B , MH1441_3B	This is one of the nervoys system tissues with elevated expression levels.
TS22: spinal cord	Present		MH1441_5B , MH1441_3B	This is one of the nervoys system tissues with elevated expression levels.