Pgam1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:125993 Cankaya M, et al., An analysis of expression patterns of genes encoding proteins with catalytic activities. BMC Genomics. 2007;8:232
Assay type:	RNA in situ
MGI Accession ID:	MGI:3769769
Gene symbol:	Pgam1
Gene name:	phosphoglycerate mutase 1

Probe:	MH1112 probe 936
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Results

3A Pgam1 ©

Specimen 3A: embryonic day 14.5 (more

)

Structure	Level	Image	Note
TS22: neocortex	Strong	3A Pgam1
TS22: midbrain	Moderate	3A Pgam1
TS22: cerebellum	Moderate	3A Pgam1
TS22: pons	Moderate	3A Pgam1
TS22: medulla oblongata	Moderate	3A Pgam1
TS22: spinal cord	Moderate	3A Pgam1
TS22: cranial ganglion	Strong	3A Pgam1
TS22: dorsal root ganglion	Strong	3A Pgam1
TS22: olfactory epithelium	Strong	3A Pgam1
TS22: intestine	Strong	3A Pgam1
TS22: salivary gland	Strong	3A Pgam1
TS22: tongue	Weak	3A Pgam1
TS22: stomach	Moderate	3A Pgam1
TS22: liver	Moderate	3A Pgam1
TS22: lung	Strong	3A Pgam1
TS22: metanephros	Strong	3A Pgam1
TS22: thyroid gland	Strong	3A Pgam1
TS22: pituitary gland	Strong	3A Pgam1
TS22: pancreas	Moderate	3A Pgam1
TS22: adrenal gland	Strong	3A Pgam1
TS22: heart	Moderate	3A Pgam1
TS22: skeletal muscle	Moderate	3A Pgam1
TS22: cartilage	Absent	3A Pgam1	There is no expression in the axial cartilage.
TS22: skeleton	Absent	3A Pgam1	There is no expression in the long bones.

MH1112_4C ©

MH1112_5C ©

Specimen MH1112: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: brain ventricular layer	Strong		MH1112_4C , MH1112_5C
TS22: cranial ganglion	Strong		MH1112_4C , MH1112_5C
TS22: dorsal root ganglion	Strong		MH1112_4C , MH1112_5C
TS22: liver	Strong		MH1112_4C , MH1112_5C
TS22: metanephros	Strong		MH1112_4C , MH1112_5C
TS22: olfactory epithelium	Strong		MH1112_4C , MH1112_5C
TS22: piriform cortex	Strong		MH1112_4C , MH1112_5C
TS22: pituitary gland	Strong		MH1112_4C , MH1112_5C
TS22: retina	Strong		MH1112_4C , MH1112_5C
TS22: salivary gland	Strong		MH1112_4C , MH1112_5C
TS22: thymus primordium	Strong		MH1112_4C , MH1112_5C
TS22: vibrissa follicle	Strong		MH1112_4C , MH1112_5C
TS22: cranial nerve	Strong	Regionally restricted	MH1112_4C , MH1112_5C	There is strong expression in the cranial nerve nuclei.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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