Probe:	MH1042 probe 905
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Weak		3A Aldob
TS22: midbrain	Weak		3A Aldob
TS22: cerebellum	Weak		3A Aldob
TS22: pons	Weak		3A Aldob
TS22: medulla oblongata	Weak		3A Aldob
TS22: spinal cord	Weak		3A Aldob
TS22: cranial ganglion	Weak		3A Aldob
TS22: dorsal root ganglion	Weak		3A Aldob
TS22: olfactory epithelium	Weak		3A Aldob
TS22: intestine	Moderate		3A Aldob
TS22: salivary gland	Weak		3A Aldob
TS22: tongue	Weak		3A Aldob
TS22: stomach	Weak		3A Aldob
TS22: liver	Strong		3A Aldob
TS22: lung	Weak		3A Aldob
TS22: metanephros	Strong		3A Aldob
TS22: thyroid gland	Weak		3A Aldob
TS22: pituitary gland	Weak		3A Aldob
TS22: pancreas	Weak		3A Aldob
TS22: adrenal gland	Weak		3A Aldob
TS22: heart	Weak		3A Aldob
TS22: skeletal muscle	Absent		3A Aldob
TS22: cartilage	Absent		3A Aldob	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Aldob	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: liver	Present		MH1042_5B
TS22: renal tubule	Present		MH1042_5B
TS22: midgut	Ambiguous		MH1042_5B	There may be weak expression in the midgut.