Probe:	MH1236 probe 1029
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Moderate		3A Aldoc
TS22: midbrain	Moderate		3A Aldoc
TS22: cerebellum	Weak		3A Aldoc
TS22: pons	Weak		3A Aldoc
TS22: medulla oblongata	Weak		3A Aldoc
TS22: spinal cord	Weak		3A Aldoc
TS22: cranial ganglion	Moderate		3A Aldoc
TS22: dorsal root ganglion	Moderate		3A Aldoc
TS22: olfactory epithelium	Weak		3A Aldoc
TS22: intestine	Weak		3A Aldoc
TS22: salivary gland	Weak		3A Aldoc
TS22: tongue	Weak		3A Aldoc
TS22: stomach	Weak		3A Aldoc
TS22: liver	Weak		3A Aldoc
TS22: lung	Weak		3A Aldoc
TS22: metanephros	Weak		3A Aldoc
TS22: thyroid gland	Weak		3A Aldoc
TS22: pituitary gland	Weak		3A Aldoc
TS22: pancreas	Weak		3A Aldoc
TS22: adrenal gland	Weak		3A Aldoc
TS22: heart	Weak		3A Aldoc
TS22: skeletal muscle	Absent		3A Aldoc
TS22: cartilage	Absent		3A Aldoc	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Aldoc	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Strong		MH1236_4A	There is strong expression in the ventricular zone of the neocortex.
TS22: hippocampus	Strong	Regionally restricted	MH1236_4A	There is strong expression in the ventricular zone.
TS22: spinal cord ventricular layer	Strong		MH1236_4A
TS22: cerebral aqueduct	Strong	Regionally restricted	MH1236_4A	There is strong expression in the neuroepithelium lining the floor of the aqueduct.
TS22: midbrain ventricular layer	Strong		MH1236_4A	There is strong expression in the neuroepithelium lining the floor of the aqueduct.
TS22: 4th ventricle	Strong	Regionally restricted	MH1236_4A	There is strong expression in the neuroepithelium lining the fourth ventricle. There is also expression in cells exiting the neuroepithelium.
TS22: hindbrain	Strong	Regionally restricted	MH1236_4A	There is strong expression in the neuroepithelium lining the fourth ventricle. There is also expression in cells exiting the neuroepithelium.
TS22: cranial ganglion	Present		MH1236_4A
TS22: dorsal root ganglion	Present		MH1236_4A
TS22: spinal cord	Present	Non-Uniform	MH1236_4A	Expression is present throughout the spinal cord; it is strong in the ventricular zone.