Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:128545
Visel A, et al., Comprehensive analysis of the expression patterns of the adenylate cyclase gene family in the developing and adult mouse brain. J Comp Neurol. 2006 Jun 10;496(5):684-97
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3768401
|
|
Gene symbol:
|
Adcy8
|
|
Gene name:
|
adenylate cyclase 8
|
|
Probe:
|
MH854 probe 828
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.
|
|
|
Results
|
Specimen
Table 2-P7:
postnatal day 7
(more  )
Specimen
Table 2-P7:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
Specimen
Table 2-P56:
postnatal day 56
(more )
Specimen
Table 2-P56:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 56
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb glomerular layer |
Strong |
|
BA106
,
HB462
|
|
| TS28: main olfactory bulb external plexiform layer |
Absent |
|
BA106
,
HB462
|
|
| TS28: main olfactory bulb mitral cell layer |
Strong |
|
BA106
,
HB462
|
|
| TS28: main olfactory bulb internal plexiform layer |
Absent |
|
BA106
,
HB462
|
|
| TS28: main olfactory bulb granule cell layer |
Absent |
|
BA106
,
HB462
|
|
| TS28: cerebral cortex |
Weak |
|
HB462
,
BA106
|
|
| TS28: hippocampus CA1 |
Moderate |
|
BA106
,
HB462
|
|
| TS28: hippocampus CA2 |
Absent |
|
HB462
,
BA106
|
|
| TS28: hippocampus CA3 |
Absent |
|
BA106
,
HB462
|
|
| TS28: dentate gyrus |
Absent |
|
HB462
,
BA106
|
|
| TS28: corpus striatum |
Absent |
|
HB462
,
BA106
|
|
| TS28: telencephalon septum |
Absent |
|
BA106
,
HB462
|
|
| TS28: amygdala |
Weak |
|
BA106
,
HB462
|
|
| TS28: thalamus |
Moderate |
|
HB462
,
BA106
|
|
| TS28: hypothalamus |
Weak |
|
HB462
,
BA106
|
|
| TS28: pretectum |
Absent |
|
HB462
,
BA106
|
|
| TS28: superior colliculus |
Weak |
|
HB462
,
BA106
|
|
| TS28: inferior colliculus |
Absent |
|
HB462
,
BA106
|
|
| TS28: midbrain tegmentum |
Weak |
|
HB462
,
BA106
|
|
| TS28: cerebellum molecular layer |
Absent |
|
HB462
,
BA106
|
|
| TS28: cerebellum Purkinje cell layer |
Absent |
|
BA106
,
HB462
|
|
| TS28: cerebellum granule cell layer |
Weak |
|
BA106
,
HB462
|
|
| TS28: pons |
Weak |
|
BA106
,
HB462
|
|
| TS28: medulla oblongata |
Weak |
|
HB462
,
BA106
|
|
Specimen
1E:
postnatal day 56
(more )
Note: This is a sagittal section.
Specimen
1E:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 56
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a sagittal section.
|
Specimen
1I:
embryonic day 14.5
(more )
Note: This is a sagittal section.
Specimen
1I:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a sagittal section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: main olfactory bulb |
Present |
Regionally restricted |
1I
|
Expression is restricted to the mantle layer.
|
Specimen
2F:
postnatal day 56
(more )
Note: This is a coronal section.
Specimen
2F:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 56
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a coronal section.
|
Specimen
2H:
embryonic day 14.5
(more )
Note: This is a sagittal section.
Specimen
2H:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a sagittal section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
2H
|
Expression is present in the rostralmost cortex in scattered cells that are grouped around the base of the nascent olfactory bulb.
|
Specimen
3K:
postnatal day 7
(more )
Note: This is a sagittal section.
Specimen
3K:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a sagittal section.
|
Specimen
3L:
postnatal day 56
(more )
Note: This is a coronal section.
Specimen
3L:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 56
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a coronal section.
|
Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thalamus |
Present |
Regionally restricted |
5E
|
Expression is sharply restricted to a dorsal subregion of the dorsal thalamus.
|
Specimen
5F:
embryonic day 14.5
(more )
Specimen
5F:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thalamus |
Present |
Regionally restricted |
5F
|
Expression is sharply restricted to a dorsal subregion of the dorsal thalamus.
|
Specimen
6B:
embryonic day 14.5
(more )
Specimen
6B:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain lateral wall |
Present |
Regionally restricted |
6B
|
Expression is present in the ventral part of the mantle layer of the midbrain roof plate. It is also expressed in more lateral parts of the mantle layer in a subset of cells.
|
| TS22: midbrain ventricular layer |
Absent |
|
6B
|
There is no expression in the neuroepithelium.
|
Specimen
6E:
embryonic day 14.5
(more )
Note: This is a sagittal section.
Specimen
6E:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This is a sagittal section.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain lateral wall |
Present |
Regionally restricted |
6E
|
Expression is present along the full length of the midbrain roof with no obvious restrocaudal gradient.
|
Specimen
Not shown:
embryonic day 14.5
(more )
Specimen
Not shown:
(close )
|
Genetic Background:
|
NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: stomach |
Present |
Regionally restricted |
|
Expression is present in some regions of the stomach mucosa.
|
|
Analysis Tools