Probe:	HB461 probe 160
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection. It is possible another probe (MGI:3724645) was used in this assay.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate		HB461
TS28: main olfactory bulb external plexiform layer	Absent		HB461
TS28: main olfactory bulb mitral cell layer	Strong		HB461
TS28: main olfactory bulb internal plexiform layer	Absent		HB461
TS28: main olfactory bulb granule cell layer	Absent		HB461
TS28: cerebral cortex	Weak		HB461
TS28: hippocampus CA1	Moderate		HB461
TS28: hippocampus CA2	Moderate		HB461
TS28: hippocampus CA3	Weak		HB461
TS28: dentate gyrus	Weak		HB461
TS28: corpus striatum	Strong		HB461
TS28: telencephalon septum	Moderate		HB461
TS28: amygdala	Moderate		HB461
TS28: thalamus	Moderate		HB461
TS28: hypothalamus	Weak		HB461
TS28: pretectum	Weak		HB461
TS28: superior colliculus	Weak		HB461
TS28: inferior colliculus	Weak		HB461
TS28: midbrain tegmentum	Weak		HB461
TS28: cerebellum molecular layer	Absent		HB461
TS28: cerebellum Purkinje cell layer	Absent		HB461
TS28: cerebellum granule cell layer	Weak		HB461
TS28: pons	Weak		HB461
TS28: medulla oblongata	Weak		HB461

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate		BA103 , HB459
TS28: main olfactory bulb external plexiform layer	Absent		BA103 , HB459
TS28: main olfactory bulb mitral cell layer	Strong		HB459 , BA103
TS28: main olfactory bulb internal plexiform layer	Absent		BA103 , HB459
TS28: main olfactory bulb granule cell layer	Absent		BA103 , HB459
TS28: cerebral cortex	Weak		HB459 , BA103
TS28: hippocampus CA1	Weak		HB459 , BA103
TS28: hippocampus CA2	Strong		HB459 , BA103
TS28: hippocampus CA3	Weak		HB459 , BA103
TS28: dentate gyrus	Weak		HB459 , BA103
TS28: corpus striatum	Strong		BA103 , HB459
TS28: telencephalon septum	Moderate		HB459 , BA103
TS28: amygdala	Weak		BA103 , HB459
TS28: thalamus	Weak		BA103 , HB459
TS28: hypothalamus	Moderate		HB459 , BA103
TS28: pretectum	Absent		HB459 , BA103
TS28: superior colliculus	Weak		BA103 , HB459
TS28: inferior colliculus	Weak		BA103 , HB459
TS28: midbrain tegmentum	Moderate		HB459 , BA103
TS28: cerebellum molecular layer	Absent		HB459 , BA103
TS28: cerebellum Purkinje cell layer	Absent		HB459 , BA103
TS28: cerebellum granule cell layer	Absent		HB459 , BA103
TS28: pons	Weak		BA103 , HB459
TS28: medulla oblongata	Weak		BA103 , HB459

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		1C
TS28: main olfactory bulb mitral cell layer	Strong		1C

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Regionally restricted	1H	Expression is restricted to the mantle layer.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		2D	Expression is present in layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer III	Present		2D	Expression is present in layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer V	Present		2D	This is one of layers with the highest expression levels.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	2K	There is strong expression in the preplate.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Uniform	3E	Expression is rather uniform.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Strong		3F

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	3P	Expression is present in the marginal zone.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Non-Uniform	4C	Expression is present in a large fraction of cells in the striatum.
TS28: accumbens nucleus	Present		4C	Expression is prominent.
TS28: caudate-putamen	Present		4C	Expression is prominent.
TS28: globus pallidus	Present		4C	Expression is much weaker than that seen in the caudate putamen and nucleus accumbens.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Non-Uniform	4I , 4F	Expression is present in a large fraction of cells in the striatum.
TS28: accumbens nucleus	Present		4F	Expression is prominent.
TS28: caudate-putamen	Present		4F	Expression is prominent.
TS28: globus pallidus	Present		4F	Expression is much weaker than that seen in the caudate putamen and nucleus accumbens.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Non-Uniform	4L	Expression is present in a large fraction of cells in the striatum.
TS28: accumbens nucleus	Present		4L	Expression is prominent.
TS28: caudate-putamen	Present		4L	Expression is prominent.
TS28: globus pallidus	Present		4L	Expression is much weaker than that seen in the caudate putamen and nucleus accumbens.

Structure	Level	Pattern	Image	Note
TS22: tectum	Present	Regionally restricted	6C	Expression is present in the developing tectum at the midline, as well as in more lateral sections. It is found predominantly in the mantle layer.