Probe:	FG26 probe 3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	4D	Expression is present in the bed nucleus.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Strong	Regionally restricted	FG26	Expresion is present in the bed nucleus of the stria terminalis.
TS22: pons	Strong	Single cells	FG26
TS22: midbrain	Weak	Regionally restricted	FG26	There is weak, ubiquitous expression in the lateral part of the midbrain.
TS22: cerebellum	Weak	Regionally restricted	FG26	There is weak, ubiquitous expression in the dorsal aspect of the cerebellum.
TS22: dorsal root ganglion	Present		FG26
TS22: vestibulo-cochlear ganglion	Present		FG26
TS22: cranial ganglion	Present	Regionally restricted	FG26	Expression is present in the acoustic ganglia but not in other cranial ganglia.
TS22: extrinsic ocular muscle	Present		FG26
TS22: intercostal muscle	Present		FG26
TS22: skeletal muscle	Present	Regionally restricted	FG26	Expression is present in mastication muscles.
TS22: musculature	Present	Regionally restricted	FG26	Expression is present in muscles surrounding the pharynx, and a thin layer of muscular tissue surrounding liver, kidney, gut, and bladder.
TS22: limb musculature	Present	Regionally restricted	FG26	Expression is present in a subpopulation of limb muscles.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted		Expression is detected in the posterior folia.