Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114758
Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3716185
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Gene symbol:
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Gdf10
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Gene name:
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growth differentiation factor 10
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Probe:
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Gdf10 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
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Results
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Specimen
6A +/+ E13.5:
embryonic day 13.5
(more  )
Specimen
6A +/+ E13.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
Regionally restricted |
6A +/+ E13.5
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Expression in the ventricular zone in presumptive Bergmann glia.
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Specimen
6A nur12 E13.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
Regionally restricted |
6A nur12 E13.5
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Expression is reduced at lateral edges of the cerebellum.
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Specimen
6A +/+ E14.5:
embryonic day 14.5
(more )
Specimen
6A +/+ E14.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
6A +/+ E14.5
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Expression in the ventricular zone in presumptive Bergmann glia.
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Specimen
6A nur12 E14.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
6A nur12 E14.5
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Expression is reduced at lateral edges of the cerebellar hemispheres.
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Specimen
6A +/+ E15.5 (left):
embryonic day 15.5
(more )
Specimen
6A +/+ E15.5 (left):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
6A +/+ E15.5 (right):
embryonic day 15.5
(more )
Specimen
6A +/+ E15.5 (right):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
6A nur12 E15.5 (left):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
6A nur12 E15.5 (left)
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Expression in posterior portion of ventricular zone, adjacent to rhombic lip, but reduced at lateral edges of the cerebellum. No expression in migrating cells in cerebellar cortex.
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Specimen
6A nur12 E15.5 (right):
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
6A nur12 E15.5 (right)
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Expression in posterior portion of ventricular zone, adjacent to rhombic lip, but reduced at lateral edges of the cerebellum. No expression in migrating cells in cerebellar cortex.
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