Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114758
Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3716164
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Gene symbol:
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Slc1a3
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Gene name:
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solute carrier family 1 (glial high affinity glutamate transporter), member 3
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Antibody:
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anti-GLAST
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
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Results
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Specimen
6B +/+ GLAST:
embryonic day 15.5
(more  )
Specimen
6B +/+ GLAST:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
6B +/+ GLAST
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Expression both in immature progenitors in the ventricular zone and in migrating glia.
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Specimen
6B nur12 GLAST:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
6B nur12 GLAST
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Authors report fewer glial cell bodies and radial fibers than in wild type in both the ventricular zone and presumptive cortex, although some radial fibers are evident.
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Analysis Tools