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Gene Expression Data
Assay Details
Assay
Reference: J:64739 Wada R, et al., Microglial activation precedes acute neurodegeneration in sandhoff disease and is suppressed by bone marrow transplantation. Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10954-9
Assay type: RT-PCR
MGI Accession ID: MGI:3697239
Gene symbol: Tnf
Gene name: tumor necrosis factor
Probe: Tnfa-pE, Tnfa-pF
Assay notes: This assay is a quantitative RT-PCR. Strength values were assigned by a GXD curator. Wild-type levels are all described as present. A two-fold increase or greater over wild-type expression is described as strong and a 10-fold increase or greater in expression is described as very strong.
Results
Image: 2f ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
1M P m 1 TS28: spinal cord Present 1 µg; total RNA Not Specified Not Specified
1M P m 1 TS28: spinal cord Present 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Hexbtm1Rlp/Hexbtm1RlpNot Specified
2M P m 2 TS28: spinal cord Present 1 µg; total RNA Not Specified Not Specified
2M P m 2 TS28: spinal cord Strong (a) 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Hexbtm1Rlp/Hexbtm1RlpNot Specified
3M P m 3 TS28: spinal cord Present 1 µg; total RNA Not Specified Not Specified
3M P m 3 TS28: spinal cord Strong (b) 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Hexbtm1Rlp/Hexbtm1RlpNot Specified
4M P m 4 TS28: spinal cord Present 1 µg; total RNA Not Specified Not Specified
4M P m 4 TS28: spinal cord Very strong (c) 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Hexbtm1Rlp/Hexbtm1RlpNot Specified
Notes:
(a) There was a 2-fold increase in relative expression level in Sandhoff disease mice compared with control mice.
(b) There was a 7-fold increase in relative expression level in Sandhoff disease mice compared with control mice.
(c) There was a 15-fold increase in relative expression level in Sandhoff disease mice compared with control mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory